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ab64227 staining mouse Testis tissue sections by immunohistochemistry (Frozen sections). Tissue was fixed in paraformaldehyde and blocked in 1% serum for 10 minutes at 20°C. The primary antibody was diluted 1/800 and incubated with sample for 20 hours at 20°C. The biotin conjugated goat polyclonal to rabbit IgG, diluted 1/500 was used as secondary.
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