Application
Flow Cytometry
Sample
Human Cell (Human dermal fibroblasts)
Permeabilization
Yes - BD Perm/Wash buffer 1X
Gating Strategy
Live cells
Specification
Human dermal fibroblasts
Preparation
Cell harvesting/tissue preparation method: Trypsin
Sample buffer: BD Perm/Wash buffer 1X
Sample buffer: BD Perm/Wash buffer 1X
Fixation
BD Cytofix/Cytoperm solution
Other product details
Incubation time
30 minute(s) · Temperature: 4°C · Diluent: BD Perm/Wash buffer 1X
Dilution
1/200
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Alexa Fluor 594 (A11005)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 594
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 06 2017