Anti-Transcription factor AP-2-alpha 抗体 (ab52222)
Key features and details
- Rabbit polyclonal to Transcription factor AP-2-alpha
- Suitable for: ELISA, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Transcription factor AP-2-alpha antibody
Transcription factor AP-2-alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Transcription factor AP-2-alpha -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Chicken -
免疫原
Synthetic peptide corresponding to Human Transcription factor AP-2-alpha.
Database link: P05549 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab52222の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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WB | (2) |
1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
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IHC-P | (2) |
Use at an assay dependent concentration.
|
特記事項 |
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ELISA
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. -
関連疾患
Defects in TFAP2A are the cause of branchiooculofacial syndrome (BOFS) [MIM:113620]; also known as branchial clefts with characteristic facies, growth retardation, imperforate nasolacrimal duct, and premature aging or lip pseudocleft-hemangiomatous branchial cyst syndrome. BOFS is a rare autosomal dominant cleft palate craniofacial disorder with variable expressivity. The major features include cutaneous anomalies, ocular anomalies, characteristic facial appearance (malformed pinnae, oral clefts), and, less commonly, renal and ectodermal (dental and hair) anomalies. -
配列類似性
Belongs to the AP-2 family. -
ドメイン
The WW-binding motif mediates interaction with WWOX. -
翻訳後修飾
Sumoylated on Lys-10; which inhibits transcriptional activity. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 395982 Chicken
- Entrez Gene: 7020 Human
- Entrez Gene: 21418 Mouse
- Entrez Gene: 306862 Rat
- Omim: 107580 Human
- SwissProt: P05549 Human
- SwissProt: P34056 Mouse
- SwissProt: P58197 Rat
see all -
別名
- Activating enhancer binding protein 2 alpha antibody
- Activating enhancer-binding protein 2-alpha antibody
- Activator protein 2 antibody
see all
画像
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All lanes : Anti-Transcription factor AP-2-alpha antibody (ab52222) at 1/500 dilution
Lane 1 : COLO 205 (Human colon adenocarcinoma cell line) extracts
Lane 2 : COLO 205 (Human colon adenocarcinoma cell line) extracts with immunizing peptide
Predicted band size: 48 kDa
Observed band size: 48 kDa -
Immunohistochemical staining of paraffin embedded human breast carcinoma tissue using ab52222.
Left: - peptide. Right: + peptide.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (27)
ab52222 は 27 報の論文で使用されています。
- Ruff KLM et al. Allele-specific transcription factor binding in a cellular model of orofacial clefting. Sci Rep 12:1807 (2022). PubMed: 35110662
- Xu H et al. Silencing of lncRNA DLEU1 inhibits tumorigenesis of ovarian cancer via regulating miR-429/TFAP2A axis. Mol Cell Biochem 476:1051-1061 (2021). PubMed: 33170430
- Fan X et al. TWIST1 and chromatin regulatory proteins interact to guide neural crest cell differentiation. Elife 10:N/A (2021). PubMed: 33554859
- Xiong Y et al. TFAP2A potentiates lung adenocarcinoma metastasis by a novel miR-16 family/TFAP2A/PSG9/TGF-ß signaling pathway. Cell Death Dis 12:352 (2021). PubMed: 33824285
- Ramamoorthy K et al. Effect of chronic alcohol exposure on gut vitamin B7 uptake: involvement of epigenetic mechanisms and effect of alcohol metabolites. Am J Physiol Gastrointest Liver Physiol 321:G123-G133 (2021). PubMed: 34077272