Anti-Transcription factor AP-2-alpha 抗体 [EPR2688(2)] (ab108311)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2688(2)] to Transcription factor AP-2-alpha
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra), IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)]
Transcription factor AP-2-alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR2688(2)] to Transcription factor AP-2-alpha -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-P, Flow Cyt (Intra), IPmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- IP: HeLa whole cell lysate; Flow Cyt (intra): JAR cells; ICC/IF: JAR cells; IHC-P: Human breast carcinoma, and mouse and rat breast tissue; WB: HeLa, C6, Mouse skin and HAP1 cell lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR2688(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108311の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (1) |
1/50.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Flow Cyt (Intra) |
1/20.
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IP |
1/20.
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特記事項 |
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ICC/IF
1/50. |
WB
1/1000 - 1/10000. Predicted molecular weight: 48 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
Flow Cyt (Intra)
1/20. |
IP
1/20. |
ターゲット情報
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機能
Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. -
関連疾患
Defects in TFAP2A are the cause of branchiooculofacial syndrome (BOFS) [MIM:113620]; also known as branchial clefts with characteristic facies, growth retardation, imperforate nasolacrimal duct, and premature aging or lip pseudocleft-hemangiomatous branchial cyst syndrome. BOFS is a rare autosomal dominant cleft palate craniofacial disorder with variable expressivity. The major features include cutaneous anomalies, ocular anomalies, characteristic facial appearance (malformed pinnae, oral clefts), and, less commonly, renal and ectodermal (dental and hair) anomalies. -
配列類似性
Belongs to the AP-2 family. -
ドメイン
The WW-binding motif mediates interaction with WWOX. -
翻訳後修飾
Sumoylated on Lys-10; which inhibits transcriptional activity. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 7020 Human
- Entrez Gene: 21418 Mouse
- Entrez Gene: 306862 Rat
- Omim: 107580 Human
- SwissProt: P05549 Human
- SwissProt: P34056 Mouse
- SwissProt: P58197 Rat
- Unigene: 519880 Human
see all -
別名
- Activating enhancer binding protein 2 alpha antibody
- Activating enhancer-binding protein 2-alpha antibody
- Activator protein 2 antibody
see all
画像
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All lanes : Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (ab108311) at 1/10000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 3 : Mouse skin lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 48 kDa -
Purified ab108311 at 1/20 dilution (0.5µg) immunoprecipitating Transcription factor AP-2-alpha in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab108311 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108311 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/10,000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 48 kDa -
Intracellular Flow Cytometry analysis of JAR (Human placenta choriocarcinoma epithelial cell) cells labeling Transcription factor AP-2-alpha with Purified ab108311 at 1/20 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Immunocytochemistry analysis of JAR (Human placenta choriocarcinoma epithelial cell) cells labeling Transcription factor AP-2-alpha with Purified ab108311 at 1/50 dilution (3.4 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat breast tissue sections labeling Transcription factor AP-2-alpha with Purified ab108311 at 1/100 dilution (1.07 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse breast tissue sections labeling Transcription factor AP-2-alpha with Purified ab108311 at 1/100 dilution (1.07 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling Transcription factor AP-2-alpha with Purified ab108311 at 1/100 dilution (1.07 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (ab108311) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TFAP2A knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDaLanes 1- 2: Merged signal (red and green). Green - ab108311 observed at 48 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab108311 was shown to react with Transcription factor AP-2-alpha in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265122 (knockout cell lysate ab257736) was used. Wild-type HeLa and TFAP2A knockout HeLa cell lysates were subjected to SDS-PAGE. ab108311 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (ab108311) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : TFAP2A (Transcription factor AP-2-alpha) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 48 kDaLanes 1 - 2: Merged signal (red and green). Green - ab108311 observed at 48 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab108311 was shown to recognize Transcription factor AP-2-alpha in wild-type HAP1 cells as signal was lost at the expected MW in TFAP2A (Transcription factor AP-2-alpha) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TFAP2A (Transcription factor AP-2-alpha) knockout samples were subjected to SDS-PAGE. Ab108311 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (22)
ab108311 は 22 報の論文で使用されています。
- Bai G et al. Function and transcriptional regulation of TCTN1 in oral squamous cell carcinoma. Oncol Rep 47:N/A (2022). PubMed: 34859261
- He C et al. Comparison of two cell-free therapeutics derived from adipose tissue: small extracellular vesicles versus conditioned medium. Stem Cell Res Ther 13:86 (2022). PubMed: 35241142
- Saito-Diaz K & Zeltner N A protocol to differentiate nociceptors, mechanoreceptors, and proprioceptors from human pluripotent stem cells. STAR Protoc 3:101187 (2022). PubMed: 35330962
- Lamontagne JO et al. Transcription factors AP-2α and AP-2β regulate distinct segments of the distal nephron in the mammalian kidney. Nat Commun 13:2226 (2022). PubMed: 35468900
- Kenny C et al. TFAP2 paralogs facilitate chromatin access for MITF at pigmentation and cell proliferation genes. PLoS Genet 18:e1010207 (2022). PubMed: 35580127