Anti-KANK2 抗体 (ab99351)
Key features and details
- Rabbit polyclonal to KANK2
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-KANK2 antibody
KANK2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KANK2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Human KANK2 aa 550-650.
Database link: Q63ZY3 -
ポジティブ・コントロール
- HeLa whole cell lysate (ab150035)
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: PBS, Tris buffered saline -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99351の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 91 kDa.
|
IP |
Use a concentration of 2 - 5 µg/ml.
|
|
IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
特記事項 |
---|
WB
1/2000 - 1/10000. Predicted molecular weight: 91 kDa. |
IP
Use a concentration of 2 - 5 µg/ml. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
-
関連性
KANK2 contains 5 ANK repeats. It is strongly expressed in cervix, colon, heart, kidney and lung. -
細胞内局在
Cytoplasm. Mitochondrion. -
参照データベース
- Entrez Gene: 25959 Human
- Omim: 614610 Human
- SwissProt: Q63ZY3 Human
-
別名
- ANKRD25 antibody
- Ankyrin repeat domain 25 antibody
- Ankyrin repeat domain containing protein 25 antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling KANK2 with ab99351 at 1/1000 (1µg/ml). Detection: DAB.
-
All lanes : Anti-KANK2 antibody (ab99351) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 91 kDa
Exposure time: 3 minutes -
Detection of Human KANK2 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded). ab99351 used for WB at 1 µg/ml and used for IP at 3 µg/mg lysate (lane 1). KANK2 was also immunoprecipitated by another rabbit anti-KANK2 antibody (lane 2) and control IgG (lane 3). Detection Chemiluminescence with exposure times of 30 seconds.
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (0)
ab99351 は論文での使用が確認できていません。