Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing (10%)
Sample
Mouse Tissue lysate - other (Brown fat)
Specification
Brown fat
Treatment
cold stress for 6 hours
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Other product details
Incubation time
1 hour(s) and 0 minute(s)
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Bands
Specific: 60 kDa
Exposure
30 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kun Dong
Verified customer
投稿 Jul 12 2013