Anti-AMCase 抗体 (ab72309)
Key features and details
- Rabbit polyclonal to AMCase
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-AMCase antibody
AMCase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to AMCase -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Zebrafish -
免疫原
Synthetic peptide corresponding to Human AMCase aa 100-200 conjugated to keyhole limpet haemocyanin. Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human AMCase.
(Peptide available asab91083) -
ポジティブ・コントロール
- This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; THP1; HepG2; MOLT4. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal lung.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72309の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 5 µg/ml.
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IHC-P | (1) |
Use a concentration of 1 µg/ml.
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
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特記事項 |
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ICC/IF
Use a concentration of 5 µg/ml. |
IHC-P
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
ターゲット情報
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機能
Degrades chitin and chitotriose. May participate in the defense against nematodes, fungi and other pathogens. Plays a role in T-helper cell type 2 (Th2) immune response. Contributes to the response to IL-13 and inflammation in response to IL-13. Stimulates chemokine production by pulmonary epithelial cells. Protects lung epithelial cells against apoptosis and promotes phosphorylation of AKT1. Its function in the inflammatory response and in protecting cells against apoptosis is inhibited by allosamidin, suggesting that the function of this protein depends on carbohydrate binding. -
組織特異性
Detected in lung epithelial cells from asthma patients (at protein level). Highly expressed in stomach. Detected at lower levels in lung. -
配列類似性
Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.
Contains 1 chitin-binding type-2 domain. -
細胞内局在
Cytoplasm and Secreted. Secretion depends on EGFR activity. - Information by UniProt
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参照データベース
- Entrez Gene: 27159 Human
- Entrez Gene: 81600 Mouse
- Entrez Gene: 113901 Rat
- GenBank: NM_021797.2 Human
- Omim: 606080 Human
- SwissProt: Q9BZP6 Human
- SwissProt: Q91XA9 Mouse
- SwissProt: Q6RY07 Rat
see all -
別名
- Acidic mammalian chitinase [Precursor] antibody
- Acidic mammalian chitinase antibody
- AMCase antibody
see all
画像
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All lanes : Anti-AMCase antibody (ab72309) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 5 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes -
ICC/IF image of ab72309 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72309, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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IHC image of AMCase staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72309, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab72309 は 4 報の論文で使用されています。
- Dong B et al. Transformation of Fonsecaea pedrosoi into sclerotic cells links to the refractoriness of experimental chromoblastomycosis in BALB/c mice via a mechanism involving a chitin-induced impairment of IFN-? production. PLoS Negl Trop Dis 12:e0006237 (2018). PubMed: 29481557
- Van Dyken SJ et al. Spontaneous Chitin Accumulation in Airways and Age-Related Fibrotic Lung Disease. Cell 169:497-509.e13 (2017). PubMed: 28431248
- Nookaew I et al. Transcriptome signatures in Helicobacter pylori-infected mucosa identifies acidic mammalian chitinase loss as a corpus atrophy marker. BMC Med Genomics 6:41 (2013). WB . PubMed: 24119614
- Maddens B et al. Chitinase-like proteins are candidate biomarkers for sepsis-induced acute kidney injury. Mol Cell Proteomics : (2012). WB ; Mouse . PubMed: 22233884