Anti-ALOX12B 抗体 (ab121785)
Key features and details
- Rabbit polyclonal to ALOX12B
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ALOX12B antibody -
製品の詳細
Rabbit polyclonal to ALOX12B -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
antigen sequence, corresponding to amino acids 423-516 (LYKLLIPHTR YTVQINSIGR AVLLNEGGLS AKGMSLGVEG FAGVMVRALS ELTYDSLYLP NDFVERGVQD LPGYYYRDDS LAVWNALEKY VTEI) of Human ALOX12B.
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ポジティブ・コントロール
- Human skin tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab121785の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Converts arachidonic acid to 12R-hydroperoxyeicosatetraenoic acid (12R-HPETE). -
組織特異性
Expressed in B-cells, hair follicles, foreskin keratinocytes and adult skin. Also expressed in psoriatic tissue. -
パスウェイ
Lipid metabolism; leukotriene D4 biosynthesis. -
関連疾患
Defects in ALOX12B are a cause of non-bullous congenital ichthyosiform erythroderma (NCIE) [MIM:242100]. NCIE is a non-bullous ichthyosis, a skin disorder characterized by abnormal cornification of the epidermis. Most affected individuals are born with a tight, shiny, translucent covering called collodion membrane. The collodion membrane subsequently evolves into generalized scaling and intense redness of the skin. Clinical features are milder than in lamellar ichthyoses and demonstrate a greater variability in the intensity of erythema, size and type of scales. In contrast to lamellar ichthyoses, scales are usually white, fine and powdery, and palms and soles are severely affected. Patients suffer from palmoplantar keratoderma, often with painful fissures, digital contractures, and loss of pulp volume. -
配列類似性
Belongs to the lipoxygenase family.
Contains 1 lipoxygenase domain.
Contains 1 PLAT domain. - Information by UniProt
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参照データベース
- Entrez Gene: 242 Human
- Omim: 603741 Human
- SwissProt: O75342 Human
- Unigene: 136574 Human
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別名
- 12R-lipoxygenase antibody
- 12R-LOX antibody
- 12R-type antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab121785 は 1 報の論文で使用されています。
- Bland PJ et al. A severe collodion phenotype in the newborn period associated with a homozygous missense mutation in ALOX12B. Br J Dermatol 173:285-7 (2015). PubMed: 25524567