Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lymphoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tampon Tris/EDTA pH8
Permeabilization
No
Specification
Lymphoma
Blocking step
No blocking step used for 5 minute(s) · Temperature: 37°C
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: Zytomed Antibody diluent
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Kit EnVision FLEX / HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
30 min of pretreatment at 98¯C, and use the Envision Flex Kit with the rabbit linker.
7 non Hodgkiniens lymphomas were tested. Signal was observed on only one of them.
3 Kits were tested but good results were only obtained with the Envision Flex Kit from DAKO.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mrs. Doris Le Béhérec
Verified customer
投稿 Dec 01 2017