Anti-ALDH2 抗体 (ab227021)
Key features and details
- Rabbit polyclonal to ALDH2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-ALDH2 antibody
ALDH2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ALDH2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow, Pig -
免疫原
Recombinant fragment within Human ALDH2 (internal sequence). The exact sequence is proprietary.
Database link: P05091 -
ポジティブ・コントロール
- ICC/IF: A549 cells. IHC-P: Mouse prostate and colon tissues; AGS xenograft tissue. WB: HepG2 whole cell and cytoplasmic extracts; GL261, C8D30, BCL-1 and RAW 264.7 whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab227021の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/100 - 1/1000.
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 56 kDa.
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ICC/IF | (2) |
1/100 - 1/1000.
|
特記事項 |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 56 kDa. |
ICC/IF
1/100 - 1/1000. |
ターゲット情報
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パスウェイ
Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2. -
配列類似性
Belongs to the aldehyde dehydrogenase family. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 508629 Cow
- Entrez Gene: 217 Human
- Entrez Gene: 11669 Mouse
- Entrez Gene: 733685 Pig
- Entrez Gene: 29539 Rat
- Omim: 100650 Human
- SwissProt: P20000 Cow
- SwissProt: P05091 Human
see all -
別名
- Acetaldehyde dehydrogenase 2 antibody
- Aldehyde dehydrogenase 2 family (mitochondrial) antibody
- Aldehyde dehydrogenase 2 family antibody
see all
画像
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All lanes : Anti-ALDH2 antibody (ab227021) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cytoplasmic extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 56 kDa10% SDS-PAGE gel.
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A549 (human lung carcinoma cell line) cells stained for ALDH2 (green) using ab227021 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin antibody diluted at 1/200 dilution.
Blue: Hoechst 33342 staining.
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Paraffin-embedded mouse prostate tissue stained for ALDH2 using ab227021 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded mouse colon tissue stained for ALDH2 using ab227021 at 1/500 dilution in immunohistochemical analysis.
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All lanes : Anti-ALDH2 antibody (ab227021) at 1/1000 dilution
Lane 1 : GL261 whole cell lysate
Lane 2 : C8D30 whole cell lysate
Lane 3 : BCL-1 whole cell lysate
Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 56 kDa7.5% SDS-PAGE gel.
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Paraffin-embedded AGS xenograft tissue stained for ALDH2 using ab227021 at 1/100 dilution in immunohistochemical analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab227021 は 1 報の論文で使用されています。
- Jin N et al. Disulfiram/copper targets stem cell-like ALDH+ population of multiple myeloma by inhibition of ALDH1A1 and Hedgehog pathway. J Cell Biochem 119:6882-6893 (2018). PubMed: 29665144