Anti-ALDH1L1 + ALDH1L2 抗体 [EPR12743(B)] - Astrocyte Marker (ab177463)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12743(B)] to ALDH1L1+ALDH1L2 - Astrocyte Marker
- Suitable for: IHC-P, WB, IHC-Fr
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker
ALDH1L1+ALDH1L2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR12743(B)] to ALDH1L1+ALDH1L2 - Astrocyte Marker -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, IHC-Frmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- HepG2, 293T, fetal brain, fetal kidney and fetal liver lysates. Human kidney and Human liver tissue. IHC-Fr: Mouse and rat liver tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR12743(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab177463の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (1) |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 99 kDa.
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IHC-Fr |
1/100.
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特記事項 |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Predicted molecular weight: 99 kDa. |
IHC-Fr
1/100. |
ターゲット情報
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細胞内局在
ALDH1L1: Cytoplasm. -
参照データベース
- Entrez Gene: 10840 Human
- Entrez Gene: 160428 Human
- Entrez Gene: 107747 Mouse
- Entrez Gene: 216188 Mouse
- Entrez Gene: 299699 Rat
- Entrez Gene: 64392 Rat
- Omim: 600249 Human
- Omim: 613584 Human
see all
画像
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ALDH1L1+ALDH1L2 with ab177463 at 1/50 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463) at 1/1000 dilution
Lane 1 : His-tagged mouse ALDH1L1 fragment recombinant protein (aa162-366)
Lane 2 : His-tagged mouse ALDH1L2 fragment recombinant protein (aa184-387)
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 99 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST
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All lanes : Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463) at 1/1000 dilution
Lane 1 : HepG2 lysate
Lane 2 : 293T cell lysate
Lane 3 : Fetal brain lysate
Lane 4 : Fetal kidney lysate
Lane 5 : Fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 99 kDa -
Immunohistochemistry (Frozen sections) analysis of mouse liver tissue labeling ALDH1L1+ALDH1L2 with ab177463 at 1/100 dilution. Tissue was fixed with 4% PFA and permeabilised with 0.2% Triton X-100. ab150077, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. DAPI (blue) was used as nucleare counterstain. Antigen retrieval was heat mediated using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).Cytoplasmic staining on mouse liver.
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Immunohistochemistry (Frozen sections) analysis of rat liver tissue labeling ALDH1L1+ALDH1L2 with ab177463 at 1/100 dilution. Tissue was fixed with 4% PFA and permeabilised with 0.2% Triton X-100. ab150077, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. DAPI (blue) was used as nucleare counterstain. Antigen retrieval was heat mediated using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).Cytoplasmic staining on rat liver.
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Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ALDH1L1+ALDH1L2 with ab177463 at 1/50 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-ALDH1L1 + ALDH1L2 antibody [EPR12743(B)] - Astrocyte Marker (ab177463) at 1/1000 dilution
Lane 1 : Fetal liver lysate at 10 µg
Lane 2 : 1X PBS (negative control)
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 99 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab177463 は 7 報の論文で使用されています。
- Zhong Y & Lan J Overexpression of Eukaryotic translation initiation factor 3D induces stem cell-like properties and metastasis in cervix cancer by activating FAK through inhibiting degradation of GRP78. Bioengineered 13:1952-1961 (2022). Human . PubMed: 35104170
- Taddei RN et al. Changes in glial cell phenotypes precede overt neurofibrillary tangle formation, correlate with markers of cortical cell damage, and predict cognitive status of individuals at Braak III-IV stages. Acta Neuropathol Commun 10:72 (2022). PubMed: 35534858
- Dobolyi A et al. Exclusive neuronal detection of KGDHC-specific subunits in the adult human brain cortex despite pancellular protein lysine succinylation. Brain Struct Funct 225:639-667 (2020). PubMed: 31982949
- Hanshaw DM et al. Large felid leucoencephalomyelopathy in a Sumatran tiger (Panthera tigris sumatrae) from an Australian zoo. Aust Vet J 97:277-282 (2019). PubMed: 31209862
- Milinkeviciute G et al. Microglia Regulate Pruning of Specialized Synapses in the Auditory Brainstem. Front Neural Circuits 13:55 (2019). PubMed: 31555101
- Peter CJ et al. In vivo epigenetic editing of Sema6a promoter reverses transcallosal dysconnectivity caused by C11orf46/Arl14ep risk gene. Nat Commun 10:4112 (2019). PubMed: 31511512
- Raddatz BB et al. Transcriptional analysis of glial cell differentiation in the postnatal murine spinal cord. Int J Dev Neurosci 42:24-36 (2015). IHC-P ; Mouse . PubMed: 25702526