Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E9.5 mouse embryo sections)
Specification
E9.5 mouse embryo sections
Fixative
Paraformaldehyde
Permeabilization
Yes - PBST (PBS/0.5%v/v TritonX100 (Sigma)
Blocking step
NOVOCASTRA protein block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
48 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA + 5% donkey serum + PBST
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 555 donkey anti-rabbit (Invitrogen)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Dilution
1/1000
Additional data
Additional Notes
This image is of a transverse section through an E9.5 embryo, showing the neural tube, somites and gut.
According to ISH, Aldh1a2 RNA is expressed in the posterior somites of E9.5 mouse embryo. Our staining result is consistent with this finding. The protein is expressed in newly formed somites and weakly in dorsal neural tube.
Antigen retrieval is not required for this staining.
According to ISH, Aldh1a2 RNA is expressed in the posterior somites of E9.5 mouse embryo. Our staining result is consistent with this finding. The protein is expressed in newly formed somites and weakly in dorsal neural tube.
Antigen retrieval is not required for this staining.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 24 2012