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Anti-ALDH1A1 antibody (ab9883)

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Overview

Product name

Anti-ALDH1A1 antibody
ALDH1A1 抗体 (13件) 一覧

Description

Goat polyclonal to ALDH1A1

Specificity

This antibody may cross-react with ALDH1A2 (GeneID 8854) with one residue difference from this design and with ALDH2 (GeneID 217) with two residues difference from this design. Please note that ALDH1A2 is known to be alternatively spliced under some conditions (37kDa) (2).

Tested applications

IHC-Fr, WB, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Predicted to work with

Cow, Dog

Immunogen

Synthetic peptide: EVKTVTVKISQKNS, corresponding to C terminal amino acids 488-501 of Human Aldehyde Dehydrogenase 1.

C-EVKTVTVK ISQKNS

Positive control

Human liver lysates.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Primary antibody notes

A protease inhibitor that inhibits fibrin clot formation.

Clonality

Polyclonal

Isotype

IgG

  • Western blot - ALDH1A1 antibody (ab9883)Western blot - ALDH1A1 antibody (ab9883) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody (ab9883)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody (ab9883) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab9883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Is unsuitable for or ICC.

Target

Function

Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid.

Pathway

Cofactor metabolism; retinol metabolism.

Sequence similarities

Belongs to the aldehyde dehydrogenase family.

Cellular localization

Cytoplasm.

Target information above from: UniProt accessionP00352 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • cytosolic antibody
  • Acetaldehyde dehydrogenase 1 antibody
  • AHD2 antibody
  • AL1A1_HUMAN antibody
  • ALDC antibody
  • Aldehyde dehydrogenase 1 family member A1 antibody
  • Aldehyde dehydrogenase 1 soluble antibody
  • Aldehyde dehydrogenase 1A1 antibody
  • Aldehyde Dehydrogenase antibody
  • Aldehyde dehydrogenase cytosolic antibody
  • Aldehyde dehydrogenase family 1 member A1 antibody
  • Aldehyde dehydrogenase liver cytosolic antibody
  • ALDH 1 antibody
  • ALDH 1A1 antibody
  • ALDH class 1 antibody
  • ALDH-E1 antibody
  • ALDH1 A1 antibody
  • ALDH1 antibody
  • ALDH11 antibody
  • ALDH1A1 antibody
  • ALHDII antibody
  • MGC2318 antibody
  • PUMB1 antibody
  • RALDH 1 antibody
  • RalDH1 antibody
  • Retinal dehydrogenase 1 antibody
see all

Anti-ALDH1A1 antibody images:

  Western blot - ALDH1A1 antibody (ab9883)

Western blot - ALDH1A1 antibody (ab9883)

Anti-ALDH1A1 antibody (ab9883) at 0.03 µg/ml + Human Liver lysate (RIPA buffer, 35µg total protein per lane).

Predicted band size : 55 kDa


Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody (ab9883)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody (ab9883)

IHC image of ab9883 staining in Human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9883, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-ALDH1A1 antibody (ab9883)

This product has been referenced in:

  • Dylla SJet al. Colorectal cancer stem cells are enriched in xenogeneic tumors following chemotherapy. PLoS ONE 3:e2428 (2008). IHC-Fr; Human.Read more (PubMed: 18560594) »
  • Yoon Met al. Developmental expression of aldehyde dehydrogenase in rat: a comparison of liver and lung development. Toxicol Sci 89:386-98 (2006).Read more (PubMed: 16291827) »

See all 4 publications for this product

Publishing research using ab9883? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"