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Western blot - ALDH1A1 antibody (ab9883)
Western blot - ALDH1A1 antibody (ab9883)
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Product Name 

ALDH1A1 antibody

 

ALDH1A1 抗体 (11件) 一覧

Product type 

Primary antibodies

Description 

Goat polyclonal to ALDH1A1

Immunogen 

Synthetic peptide: EVKTVTVKISQKNS, corresponding to C terminal amino acids 488-501 of Human Aldehyde Dehydrogenase 1.
BLAST 'C-EVKTVTVKISQKNS' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 

Hu

Predicted to react 
(species key)

Cow
(Due to sequence homology)

Specificity 

This antibody may cross-react with ALDH1A2 (GeneID 8854) with one residue
difference from this design and with ALDH2 (GeneID 217) with two residues
difference from this design.

Please note that ALDH1A2 is known to be alternatively spliced under some conditions (37kDa) (2).

Tested applications 
(see key)

IHC-Fr, IHC-P, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
IHC-P: 1/75. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. (PubMed: 16982809)
IHC-Fr: Use at an assay dependent dilution. (PubMed: 18560594)
WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 55 kDa.

Is unsuitable for ICC.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human liver lysates.

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Tumor biomarkers >> Other
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other

Relevance 

ALDH1A1 is an aldehyde dehydrogenase able to oxidize a wide variety of aliphatic aldehydes (retinaldehyde, acetaldehyde, etc.) to the corresponding carboxylic acids (retinoic acid, acetic acid, etc.). ALDH1A1 (also known as RALDH1, ALDH1, or AHD2) is highly expressed in the dorsal retina, ventral midbrain (dopaminergic neurons), and hematopoietic stem cells. ALDH1A1 is involved in retinoic acid (RA) synthesis during vertebrate embryogenesis. ALDH1A1 is first detected at E9.0-E10.5 in cranial tissues (ventral mesencephalon, dorsal retina, thymic primordia, optic vesicles) and in the mesonephros. ALDH1A1 is also of interest in Parkinson's Disease (PD) being expressed in the the A9 dopaminergic (DA) neuronal group projecting to the dorsal striatum; this is the most vulnerable site in PD (Chung et al, 2005). Some studies also identify ALDH1A1 as a marker of cancer stem cells. Studies of breast cancer tissues showed that those that expressed a form of ALDH1A1 had the worst outcomes.

A protease inhibitor that inhibits fibrin clot formation.

 

ALDH1A1 antibody (ab9883)の別名

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

281615    (Cow)
216    (Human)
11668    (Mouse)
24188    (Rat)

Omim

    

100640    (Human)

SwissProt

    

P48644    (Cow)
P00352    (Human)
P24549    (Mouse)
P51647    (Rat)

Unigene

    

76392    (Human)
6132    (Rat)

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

この製品は、毒物及び劇物取締法に基づく「毒物」に該当します。

Purification notes 

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

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See below for ALDH1A1 antibody images, references, products related to ab9883 and other tools.

 

ALDH1A1 antibody images:

Product image  Western blot - ALDH1A1 antibody (ab9883)

Western blot - ALDH1A1 antibody (ab9883)

ALDH1A1 antibody (ab9883) at 0.03 µg/ml + Human Liver lysate (RIPA buffer, 35µg total protein per lane).

Predicted band size : 55 kDa


Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

 

References for ALDH1A1 antibody (ab9883)

Specific publication references   This product has been used in: (two most recent references)

Dylla SJ et al. Colorectal cancer stem cells are enriched in xenogeneic tumors following chemotherapy. PLoS ONE 3:e2428 (2008). IHC-Fr; Human. PubMed: 18560594

Yoon M et al. Developmental expression of aldehyde dehydrogenase in rat: a comparison of liver and lung development. Toxicol Sci 89:386-98 (2006). PubMed: 16291827


See all 4 publication references for this product.


If you publish research using ab9883 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to ALDH1A1

 

ALDH1A1 antibody - more information

Customer reviews (feedback) regarding ALDH1A1 antibody

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Protocols for ALDH1A1 antibody

Price and availability of products related to ALDH1A1 antibody

 

ALDH1A1 antibody - related products:

Products for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Slides

Products for Western blot

Loading controls

Lysates

 

Immunizing Peptide for ALDH1A1 antibody

ab23169

  

ALDH1A1 peptide (488-501)

 

Positive Controls for ALDH1A1 antibody

ab7902

  

HEK293 Human embryonic kidney cell line Whole Cell Lysate

ab7937

  

Spleen (Mouse) Whole Cell Lysate - normal tissue

 

Compatible Secondaries for ALDH1A1 antibody

ab6566

  

Cy5 ® antibody

ab6739

  

Texas Red ® antibody

ab6740

  

Biotin antibody

ab6741

  

HRP antibody

ab6742

  

Alkaline Phosphatase antibody

ab6881

  

FITC antibody

ab7004

  

Phycoerythrin antibody

ab27245

  

10nm Gold antibody

ab27246

  

10nm Gold antibody

ab27247

  

15nm Gold antibody

 

Other ALDH1A1 Antibodies

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ALDH1A1 antibody - other tools:

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