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Western blot - ALDH1A1 antibody (ab9883)
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Product Name
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ALDH1A1 antibody
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ALDH1A1 抗体 (11件) 一覧
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Product type
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Primary antibodies
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Description
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Goat polyclonal to ALDH1A1
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Immunogen
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Synthetic peptide: EVKTVTVKISQKNS, corresponding to C terminal amino acids 488-501 of Human Aldehyde Dehydrogenase 1.
BLAST 'C-EVKTVTVKISQKNS' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Cow
(Due to sequence homology)
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Specificity
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This antibody may cross-react with ALDH1A2 (GeneID 8854) with one residue
difference from this design and with ALDH2 (GeneID 217) with two residues
difference from this design.
Please note that ALDH1A2 is known to be alternatively spliced under some conditions (37kDa) (2).
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Tested applications
(see key)
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IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: 1/75. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. (PubMed: 16982809)
IHC-Fr: Use at an assay dependent dilution. (PubMed: 18560594)
WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 55 kDa.
Is unsuitable for ICC.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human liver lysates.
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Tumor biomarkers >> Other
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
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Relevance
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ALDH1A1 is an aldehyde dehydrogenase able to oxidize a wide variety of aliphatic aldehydes (retinaldehyde, acetaldehyde, etc.) to the corresponding carboxylic acids (retinoic acid, acetic acid, etc.). ALDH1A1 (also known as RALDH1, ALDH1, or AHD2) is highly expressed in the dorsal retina, ventral midbrain (dopaminergic neurons), and hematopoietic stem cells. ALDH1A1 is involved in retinoic acid (RA) synthesis during vertebrate embryogenesis. ALDH1A1 is first detected at E9.0-E10.5 in cranial tissues (ventral mesencephalon, dorsal retina, thymic primordia, optic vesicles) and in the mesonephros. ALDH1A1 is also of interest in Parkinson's Disease (PD) being expressed in the the A9 dopaminergic (DA) neuronal group projecting to the dorsal striatum; this is the most vulnerable site in PD (Chung et al, 2005). Some studies also identify ALDH1A1 as a marker of cancer stem cells. Studies of breast cancer tissues showed that those that expressed a form of ALDH1A1 had the worst outcomes.
A protease inhibitor that inhibits fibrin clot formation.
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ALDH1A1 antibody (ab9883)の別名 |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
この製品は、毒物及び劇物取締法に基づく「毒物」に該当します。
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Abcam では全ての製品情報を一元管理しており、 ALDH1A1 antibody について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。 |
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See below for ALDH1A1 antibody images, references, products related to ab9883 and other tools.
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ALDH1A1 antibody images:
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 Western blot - ALDH1A1 antibody (ab9883)
ALDH1A1 antibody (ab9883) at 0.03 µg/ml + Human Liver lysate (RIPA buffer, 35µg total protein per lane).
Predicted band size : 55 kDa
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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References for ALDH1A1 antibody (ab9883)
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This product has been used in: (two most recent references)
Dylla SJ et al. Colorectal cancer stem cells are enriched in xenogeneic tumors following chemotherapy. PLoS ONE 3:e2428 (2008). IHC-Fr; Human. PubMed: 18560594
Yoon M et al. Developmental expression of aldehyde dehydrogenase in rat: a comparison of liver and lung development. Toxicol Sci 89:386-98 (2006). PubMed: 16291827
See all 4 publication references for this product.
If you publish research using ab9883 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to ALDH1A1
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ALDH1A1 antibody - more information
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