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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Overview
Product name
Anti-ALDH1A1 antibody
ALDH1A1 抗体 (13件) 一覧
Description
Goat polyclonal to ALDH1A1
Specificity
This antibody may cross-react with ALDH1A2 (GeneID 8854) with one residue difference from this design and with ALDH2 (GeneID 217) with two residues difference from this design. Please note that ALDH1A2 is known to be alternatively spliced under some conditions (37kDa) (2).
Tested applications
IHC-Fr, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Cow, Dog
Immunogen
Synthetic peptide: EVKTVTVKISQKNS, corresponding to C terminal amino acids 488-501 of Human Aldehyde Dehydrogenase 1.
C-EVKTVTVK ISQKNS
Positive control
Human liver lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Primary antibody notes
A protease inhibitor that inhibits fibrin clot formation.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Other
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution. (PubMed: 18560594)
WB: Use a concentration of 0.3 - 1 µg/ml.Predicted molecular weight: 55 kDa.
IHC-P: Use a concentration of 5 µg/ml
Application notes
Is unsuitable for or ICC.
Target
Function
Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid.
Pathway
Cofactor metabolism; retinol metabolism.
Sequence similarities
Belongs to the aldehyde dehydrogenase family.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP00352
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- cytosolic antibody
- Acetaldehyde dehydrogenase 1 antibody
- AHD2 antibody
see all
Database links
- Entrez Gene: 281615 Cow
- Entrez Gene: 216 Human
- Entrez Gene: 11668 Mouse
- Entrez Gene: 24188 Rat
- Omim: 100640 Human
- SwissProt: P48644 Cow
- SwissProt: P00352 Human
- SwissProt: P24549 Mouse
see all
Anti-ALDH1A1 antibody images:
Western blot - ALDH1A1 antibody (ab9883)
Anti-ALDH1A1 antibody (ab9883) at 0.03 µg/ml + Human Liver lysate (RIPA buffer, 35µg total protein per lane).
Predicted band size : 55 kDa
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A1 antibody (ab9883)
IHC image of ab9883 staining in Human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9883, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-ALDH1A1 antibody (ab9883)
This product has been referenced in:
- Dylla SJet al. Colorectal cancer stem cells are enriched in xenogeneic tumors following chemotherapy. PLoS ONE 3:e2428 (2008). IHC-Fr; Human.Read more (PubMed: 18560594) »
- Yoon Met al. Developmental expression of aldehyde dehydrogenase in rat: a comparison of liver and lung development. Toxicol Sci 89:386-98 (2006).Read more (PubMed: 16291827) »
See all 4 publications for this product
Publishing research using ab9883? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"