Application
Western blot
Loading amount
75 µg
Gel Running Conditions
Reduced Denaturing (10% Tris-Glycine)
Sample
Human Cell lysate - whole cell (HEK-293T)
Specification
HEK-293T
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in TBS-T
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL Femto
Bands
Specific: 55 kDa
Exposure
30 second(s)
Positive control
HEK-293T cell lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 11 2015