Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (12% acrylamide gel)
Sample
Human Tissue lysate - whole (Brain)
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking agent
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Rabbit IgG (H+L), HRP conjugate
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000
Detection
Detection method
ECL
Bands
Specific: 55 kDa
Exposure
30 second(s)
Positive control
Rat liver tissue
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Sep 24 2013