![Western blot - ALDH1A1 antibody [EP1933Y] (ab52492) image](#52492 WB.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ALDH1A1 antibody [EP1933Y] (ab52492) image](#52492 IHC.jpg)
![Immunocytochemistry/ Immunofluorescence - ALDH1A1 antibody [EP1933Y] (ab52492) image](#ALDH1A1-Primary-antibodies-ab52492-1.jpg)
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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Overview
Product name
Anti-ALDH1A1 antibody [EP1933Y]
ALDH1A1 抗体 (13件) 一覧
Description
Rabbit monoclonal [EP1933Y] to ALDH1A1
Tested applications
ICC/IF, WB, IP, ICC, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to residues near the C-terminus of human Aldh1a1
Positive control
HepG2 cell lysate; human liver tissue
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP1933Y
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Other
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent concentration. (PubMed: 21396117)
WB: 1/2000Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IP
IP: 1/30IP: 1/30
ICC
ICC: 1/100 - 1/250.ICC: 1/100 - 1/250.
IHC-P: 1/100 - 1/100.(We have observed batch to batch variability in the recommended dilution. Please titrate each new batch over a range of dilutions. (An antigen retrieval step is recommended))
Application notes
Is unsuitable for or Flow Cyt.
Target
Function
Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid.
Pathway
Cofactor metabolism; retinol metabolism.
Sequence similarities
Belongs to the aldehyde dehydrogenase family.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP00352
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- cytosolic antibody
- Acetaldehyde dehydrogenase 1 antibody
- AHD2 antibody
see all
Database links
- Entrez Gene: 216 Human
- Entrez Gene: 11668 Mouse
- Entrez Gene: 24188 Rat
- Omim: 100640 Human
- SwissProt: P00352 Human
- SwissProt: P24549 Mouse
- SwissProt: P51647 Rat
- Unigene: 76392 Human
see all
Anti-ALDH1A1 antibody [EP1933Y] images:
Western blot - ALDH1A1 antibody [EP1933Y] (ab52492)
![Western blot - ALDH1A1 antibody [EP1933Y] (ab52492)](/ps/datasheet/Images/52/ab52492/52492 WB.jpg)
Anti-ALDH1A1 antibody [EP1933Y] (ab52492) at 1/2000 dilution + HepG2 cell lysate at 10 µg
Secondary
HRP labeled Goat anti-rabbit at 1/2000 dilution
Predicted band size : 55 kDa
Observed band size : 55 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ALDH1A1 antibody [EP1933Y] (ab52492)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ALDH1A1 antibody [EP1933Y] (ab52492)](/ps/datasheet/Images/52/ab52492/52492 IHC.jpg)
Immunohistochemical analysis of paraffin-embedded human liver using ab52492 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - ALDH1A1 antibody [EP1933Y] (ab52492)
![Immunocytochemistry/ Immunofluorescence - ALDH1A1 antibody [EP1933Y] (ab52492)](/ps/datasheet/images/52/ab52492/ALDH1A1-Primary-antibodies-ab52492-1.jpg)
ICC/IF image of ab52492 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52492, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-ALDH1A1 antibody [EP1933Y] (ab52492)
This product has been referenced in:
- Li Wet al. Mitochondrion as a novel site of dichloroacetate biotransformation by glutathione transferase zeta1. J Pharmacol Exp Ther 336:87-94 (2011). WB; Human.Read more (PubMed: 20884751) »
- Burga LNet al. Loss of BRCA1 leads to an increase in epidermal growth factor receptor expression in mammary epithelial cells, and epidermal growth factor receptor inhibition prevents estrogen receptor-negative cancers in BRCA1-mutant mice. Breast Cancer Res 13:R30 (2011). ICC/IF; Human.Read more (PubMed: 21396117) »
See all 7 publications for this product
Publishing research using ab52492? Please let us know so that we can cite the reference in this datasheet
![ALDH1A1 antibody [EP1933Y] for Western blot in Mouse (52492)](/ps/datasheet/images/52/ab52492/ALDH1A1-Primary-antibodies-ab52492-3.JPG)
![ALDH1A1 antibody [EP1933Y] for IHC-P in Mouse (52492)](/ps/Reviews/Images/ab11843_62575.jpg)
![Anti-ALDH1A1 antibody [EP1933Y] for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Mouse (52492)](/ps/datasheet/images/52/ab52492/ALDH1A1-Primary-antibodies-ab52492-7.JPG)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"