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ab52492 has been referenced in 7 publications.
Publishing research using ab52492? Please let us know so that we can cite the reference in this datasheet
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Anti-ALDH1A1 antibody [EP1933Y] (ab52492) at 1/2000 dilution + HepG2 cell lysate at 10 µg
Secondary
HRP labeled Goat anti-rabbit at 1/2000 dilution
Predicted band size : 55 kDa
Observed band size : 55 kDa
Immunohistochemical analysis of paraffin-embedded human liver using ab52492 at 1/100 dilution.
ICC/IF image of ab52492 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52492, 1/1000 dilution) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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