Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Sample
Pig Cell (PAE cells)
Specification
PAE cells
Permeabilization
Yes - 0.1% Tween
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Diluent: 3% BSA - PBS
Dilution
1/50
Secondary antibody
Name
Non-Abcam antibody was used: anti Rb
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Dilution
1/500
Additional data
Additional Notes
Although previous results (IHC, WB) from our lab shows nuclear P-Akt2 using this antibody, we can't discard the possibility that our protocol skip nuclear detection.
Abcam response
Thank you for your valuable feedback. We have not previously tested this antibody on pig so it may not be suitable for use in this species. We welcome other abreviews to determine this.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 May 12 2014