Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse mammary cell)
Loading amount
100 µg
Specification
Mouse mammary cell
Treatment
5ng/ml TGF beta for different time periods
Gel Running Conditions
Reduced Denaturing (10.0% gel)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/500
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5 %FBS in TTBS
Secondary antibody
Name
Non-Abcam antibody was used: GE anti-rabbit HRP
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
GE ECL plus
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 62 kDa Non-specific: 50, 40, 30, 10 kDa
Positive control
0,1,3,6,12 and 24hrs TGF beta treated MME lysate
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投稿 Aug 31 2011