Goat polyclonal to AKAP12
, corresponding to C terminal amino acids 1769-1782 of Human AKAP12(Peptide available as
Jurkat and HeLa cell lysates
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Tris saline, 0.02% Na azide, pH7.3 with 0.5% BSA
Concentration information loading...
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunizing Peptide (Blocking)
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 268 kDa (predicted molecular weight: 196 kDa).Can be blocked with
Human AKAP12 peptide (ab23159).
Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).
Expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells.
Contains 3 AKAP domains.
Polybasic regions located between residues 266 and 557 are involved in binding PKC.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. May be part of the cortical cytoskeleton.
Information by UniProt
A kinase (PRKA) anchor protein (gravin) 12 antibody
A kinase Anchor Protein 12 antibody
A kinase anchor protein 250kDa antibody
Western blot - Anti-AKAP12 antibody (ab9698)
All lanes :
Anti-AKAP12 antibody (ab9698) at 1 µg/ml
Lane 1 :
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (
Lane 2 : SW480 whole cell lysate ( ab3957) Lysates/proteins at 20 µg per lane. Secondary All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) ( ab65486) at 1/3000 dilution Performed under reducing conditions. Predicted band size: 196 kDa Observed band size: 268 kDa ( why is the actual band size different from the predicted?) Additional bands at: 100 kDa, 30 kDa, 45 kDa. We are unsure as to the identity of these extra bands.
AKAP12 contains a number of potential phosphorylation sites (SwissProt data) which may explain it running at a higher molecular weight than predicted. The 268 kDa band is comparable to molecular weights seen with other commercially available antibodies to AKAP12.
This product has been referenced in:
et al. RNA silencing identifies PDE4D5 as the functionally relevant cAMP phosphodiesterase interacting with beta arrestin to control the protein kinase A/AKAP79-mediated switching of the beta2-adrenergic receptor to activation of ERK in HEK293B2 cells. J Biol Chem 280:33178-89 (2005).
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