Anti-AICDA 抗体 - ChIP Grade (ab5197)
Key features and details
- Rabbit polyclonal to AICDA - ChIP Grade
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-AICDA antibody - ChIP Grade
AICDA 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to AICDA - ChIP Grade -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab13718) -
ポジティブ・コントロール
- This antibody gave a positive signal in AICDA 293T Cell Transient Overexpression Lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5197の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).Can be blocked with Human AICDA peptide (ab13718).
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).Can be blocked with Human AICDA peptide (ab13718). |
ターゲット情報
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機能
RNA-editing deaminase involved in somatic hypermutation, gene conversion, and class-switch recombination. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. -
組織特異性
Strongly expressed in lymph nodes and tonsils. -
関連疾患
Defects in AICDA are the cause of hyper-IgM immunodeficiency syndrome type 2 (HIGM2) [MIM:605258]; also known as hyper-IgM syndrome 2. HIGM2 is an autosomal recessive disorder characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE, resulting in a profound susceptibility to bacterial infections. HIGM2 causes the absence of Ig class switch recombination (CSR), the lack of Ig somatic hypermutations, and lymph node hyperplasia caused by the presence of giant germinal centers. -
配列類似性
Belongs to the cytidine and deoxycytidylate deaminase family. - Information by UniProt
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参照データベース
- Entrez Gene: 57379 Human
- Entrez Gene: 11628 Mouse
- Omim: 605257 Human
- SwissProt: Q9GZX7 Human
- SwissProt: Q9WVE0 Mouse
- Unigene: 149342 Human
- Unigene: 391503 Mouse
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別名
- Activation induced cytidine deaminase antibody
- Activation induced deaminase antibody
- Activation-induced cytidine deaminase antibody
see all
画像
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Anti-AICDA antibody - ChIP Grade (ab5197) at 1 µg/ml + AICDA 293T Cell Transient Overexpression Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab5197 は 2 報の論文で使用されています。
- Dayal S et al. High resolution analysis of the chromatin landscape of the IgE switch region in human B cells. PLoS One 6:e24571 (2011). ChIP ; Human . PubMed: 21949728
- Duquette ML et al. AID binds to transcription-induced structures in c-MYC that map to regions associated with translocation and hypermutation. Oncogene 24:5791-8 (2005). PubMed: 15940261