Anti-AGXT 抗体 [1F9BC7] (ab110313)
Key features and details
- Mouse monoclonal [1F9BC7] to AGXT
- Suitable for: ICC/IF, IP
- Reacts with: Cow, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-AGXT antibody [1F9BC7]
AGXT 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [1F9BC7] to AGXT -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, IPmore details -
種交差性
交差種: Cow, Human -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- HepG2 cells; Human and Rat liver, Bovine heart and HepG2 cell lysates.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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特記事項(精製)
ab110313 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity: >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
1F9BC7 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab110313の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 5 µg/ml.
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IP |
Use at an assay dependent concentration.
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特記事項 |
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ICC/IF
Use a concentration of 5 µg/ml. |
IP
Use at an assay dependent concentration. |
ターゲット情報
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組織特異性
Liver. -
関連疾患
Defects in AGXT are the cause of hyperoxaluria primary type 1 (HP1) [MIM:259900]; also known as primary hyperoxaluria type I (PH1) and oxalosis I. HP1 is a rare autosomal recessive inborn error of glyoxylate metabolism characterized by increased excretion of oxalate and glycolate, and the progressive accumulation of insoluble calcium oxalate in the kidney and urinary tract. -
配列類似性
Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. -
細胞内局在
Peroxisome. Mitochondrion matrix. Except in some HP1 patients where AGT is found in the mitochondrial matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 189 Human
- Omim: 604285 Human
- SwissProt: P21549 Human
- Unigene: 144567 Human
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別名
- AGT antibody
- AGT1 antibody
- Agxt antibody
see all
画像
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Immunocytochemistry analysis using ab110313 at 5 ug/ml staining AGXT in HepG2 cells (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (green).
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Using MitoSciences' standard immunoprecipitation protocol, ab110313 precipicates with the 43kDa AGXT protein in human liver samples. Very small amounts of AGXT are immunoprecipitated from bovine heart, rat liver or human HepG2 cultured cell lysate. Identity of this protein was confirmed by mass spectrometry. This gel was stained with silver.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab110313 は論文での使用が確認できていません。