Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
kidney
Blocking step
Blocking reagens Fa.Zytomed as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: antibody solution Fa.Zytomed
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: ABC Fa.Zytomed
Clonality: Polyclonal
Conjugation: Biotin
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Broad spectrum streptavidin-biotin system used for detection. Able to detect primary antibodies raised in mouse, rabbit, rat and guinea pig.
Abcam response
Thank you for submitting this review. We have as yet not received any other reports with this antibody being used with mouse samples. As the homology is 76% between the mouse protein and the human protein against which this antibody was raised, the antibody may not cross react. We would welcome any reports from customers who have tried the antibody with rabbit samples, on in IHC.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Mar 01 2013