Anti-RAB 抗体 (ab86349)
Key features and details
- Rabbit polyclonal to RAB
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RAB antibody
RAB 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RAB -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis -
免疫原
Synthetic peptide corresponding to Human RAB aa 512-562.
Database link: NP_004495.2 -
ポジティブ・コントロール
- HeLa or 293T cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86349の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/2000 - 1/10000. Predicted molecular weight: 58 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 58 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Required for vesicle docking or fusion during acrosome biogenesis (By similarity). May play a role in RNA trafficking or localization. In case of infection by HIV-1, acts as a cofactor for viral Rev and promotes movement of Rev-responsive element-containing RNAs from the nuclear periphery to the cytoplasm. This step is essential for HIV-1 replication. -
組織特異性
Ubiquitously expressed. -
配列類似性
Contains 1 Arf-GAP domain. -
ドメイン
Contains FG repeats. The FG repeat region is required for acting as a cofactor of HIV-1 Rev. -
翻訳後修飾
O-glycosylated. -
細胞内局在
Nucleus. Cytoplasmic vesicle. - Information by UniProt
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参照データベース
- Entrez Gene: 459990 Chimpanzee
- Entrez Gene: 536149 Cow
- Entrez Gene: 101134648 Gorilla
- Entrez Gene: Human
- Entrez Gene: 3267 Human
- Entrez Gene: 363266 Rat
- Entrez Gene: 708910 Rhesus monkey
- Omim: 600862 Human
see all -
別名
- AGFG1 antibody
- AGFG1_HUMAN antibody
- Arf-GAP domain and FG repeats-containing protein 1 antibody
see all
画像
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All lanes : Anti-RAB antibody (ab86349) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 58 kDa
Exposure time: 10 seconds -
Detection of RAB by Western Blot of Immunprecipitate.
ab86349 at 1µg/ml staining RAB in HeLa whole cell lysate immunoprecipitated using ab86349 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab86349 は 2 報の論文で使用されています。
- Fujioka K et al. Wheat Germ Agglutinin (WGA)-SDS-PAGE: A Novel Method for the Detection of O-GlcNAc-modified Proteins by Lectin Affinity Gel Electrophoresis. Bio Protoc 8:e3098 (2018). PubMed: 34532545
- Kubota Y et al. WGA-based lectin affinity gel electrophoresis: A novel method for the detection of O-GlcNAc-modified proteins. PLoS One 12:e0180714 (2017). Human . PubMed: 28686627