Anti-AFM 抗体 (ab152141)
Key features and details
- Rabbit polyclonal to AFM
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-AFM antibody
AFM 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to AFM -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 383-617 of Human AFM.
-
ポジティブ・コントロール
- 293T, A431, HeLa, HepG2, NIH 3T3, JC and BCL-1 whole cell lysates; Cal27 xenograft tissue; HeLa cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab152141の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 69 kDa.
|
|
IHC-P |
1/100 - 1/1000.
|
|
ICC/IF |
1/100 - 1/1000.
|
特記事項 |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 69 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
ターゲット情報
-
機能
Vitamin E binding protein. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient or across the blood-brain barrier. -
配列類似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
翻訳後修飾
N-glycosylated. -
細胞内局在
Secreted. - Information by UniProt
-
参照データベース
- Entrez Gene: 173 Human
- Entrez Gene: 280662 Mouse
- Omim: 104145 Human
- SwissProt: P43652 Human
- SwissProt: O89020 Mouse
- Unigene: 168718 Human
- Unigene: 348786 Mouse
-
別名
- AFAM_HUMAN antibody
- Afamin antibody
- Afm antibody
see all
画像
-
All lanes : Anti-AFM antibody (ab152141) at 1/5000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 69 kDa
7.5% SDS PAGE -
All lanes : Anti-AFM antibody (ab152141) at 1/5000 dilution
Lane 1 : NIH 3T3 whole cell lysate
Lane 2 : JC whole cell lysate
Lane 3 : BCL-1 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 69 kDa
7.5% SDS PAGE -
Immunofluorescent analysis of methanol-fixed HeLa cells labeling AFM with ab152141 at 1/500 dilution. Lower image co-stained with Hoerchst 33342.
-
Immunohistochemical analysis of paraffin-embedded Cal27 (human) xenograft labeling AFM with ab152141 at 1/500 dilution.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab152141 は論文での使用が確認できていません。