Anti-AFM 抗体 [EPR19383] (ab201034)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19383] to AFM
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-AFM antibody [EPR19383]
AFM 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR19383] to AFM -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Untreated and PNGase F treated mouse serum; Human serum, Human blood, mouse plasma, mouse serum, rat serum, rat plasma and Human plasma.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR19383 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab201034の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 69 kDa).
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特記事項 |
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WB
1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 69 kDa). |
ターゲット情報
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機能
Vitamin E binding protein. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient or across the blood-brain barrier. -
配列類似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
翻訳後修飾
N-glycosylated. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 173 Human
- Entrez Gene: 280662 Mouse
- Entrez Gene: 282708 Rat
- Omim: 104145 Human
- SwissProt: P43652 Human
- SwissProt: O89020 Mouse
- SwissProt: P36953 Rat
- Unigene: 168718 Human
see all -
別名
- AFAM_HUMAN antibody
- Afamin antibody
- Afm antibody
see all
画像
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All lanes : Anti-AFM antibody [EPR19383] (ab201034) at 1/1000 dilution
Lane 1 : Human serum at 20 µg
Lane 2 : Human blood at 20 µg
Lane 3 : Mouse plasma at 20 µg
Lane 4 : Mouse serum at 20 µg
Lane 5 : Rat serum at 20 µg
Lane 6 : Rat plasma at 20 µg
Lane 7 : Human plasma at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 69 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1,2,3,4,5 and 7: 30 seconds; Lane 6: 3 seconds.
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All lanes : Anti-AFM antibody [EPR19383] (ab201034) at 1/1000 dilution
Lane 1 : Untreated mouse serum
Lane 2 : Mouse serum PNGase F treated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 69 kDa
Observed band size: 65,87 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
AFM is a glycoprotein, its apparent molecular weight on SDS-PAGE is 87 kDa due to glycosylation. AFM has 4 potential sites for N-Glycosylation and N-glycanase treatment reduced the apparent MW to 65 kDa (PMID:7517938).
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab201034 は論文での使用が確認できていません。