製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ADH5
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: IHC-P, WB, Flow Cytmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit
  • 免疫原

    Synthetic peptide within Human ADH5 aa 212-239 (internal sequence) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P11766

  • ポジティブ・コントロール
    • IHC-P: Human breast and placenta tissue. WB: K562 cell lysate; ADH5 transfected HEK-293 cell lysate. Flow Cytometry: K562 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab230871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/10 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 40 kDa.
Flow Cyt 1/10 - 1/50.

ターゲット情報

  • 機能
    Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione.
  • 配列類似性
    Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ADH 3 antibody
    • ADH5 antibody
    • ADHX antibody
    • ADHX_HUMAN antibody
    • Alcohol dehydrogenase (class III) chi polypeptide antibody
    • alcohol dehydrogenase 5 (class III) chi polypeptide antibody
    • Alcohol dehydrogenase 5 antibody
    • Alcohol dehydrogenase class 3 antibody
    • Alcohol dehydrogenase class chi chain antibody
    • Alcohol dehydrogenase class III antibody
    • Alcohol dehydrogenase class-3 antibody
    • Alcohol dehydrogenase class-III antibody
    • class III alcohol dehydrogenase 5 chi subunit antibody
    • FALDH antibody
    • FDH antibody
    • formaldehyde dehydrogenase antibody
    • Glutathione dependent formaldehyde dehydrogenase antibody
    • Glutathione-dependent formaldehyde dehydrogenase antibody
    • GSH-FDH antibody
    • GSNOR antibody
    • hydroxymethyllutathione dehydrogenase antibody
    • S-(hydroxymethyl)glutathione dehydrogenase antibody
    see all

画像

  • Formalin-fixed, paraffin-embedded human breast tissue stained for ADH5 with ab230871 at 1/10 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human placenta tissue stained for ADH5 with ab230871 at 1/10 dilution in immunohistochemical analysis.

  • Anti-ADH5 antibody (ab230871) at 1/1000 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 40 kDa

  • All lanes : Anti-ADH5 antibody (ab230871) at 1/1000 dilution

    Lane 1 : Non-transfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : ADH5 transfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

    Lysates/proteins at 2 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 40 kDa

  • Flow Cytometry analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells, labeling ADH5 with ab230871 at a 1/10 dilution (green) compared to a negative control (blue).

    FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

参考文献

ab230871 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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