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Synthetic peptide within Human ADH1B aa 216-245 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: P00325
Our Abpromise guarantee covers the use of ab175515 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/10 - 1/50.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000. Predicted molecular weight: 40 kDa.|
Immunofluorescence analysis of HepG2 cells labeling ADH1B with ab175515 at a 1/10 dilution, followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Immunohistochemical analysis of formalin fixed, paraffin-embedded Human hepatocarcinoma tissue labeling ADH1B with ab175515 at a 1/50 dilution, followed by DAB staining.
Flow cytometry analysis of HepG2 cells labeling ADH1B with ab175515 at 1/10 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ab175515 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"