製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ADH1B
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ICC/IF, Flow Cyt, IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chicken
  • 免疫原

    Synthetic peptide within Human ADH1B aa 216-245 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P00325

  • ポジティブ・コントロール
    • Human hepatocarcinoma tissue, HepG2 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab175515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/10 - 1/50.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 40 kDa.

ターゲット情報

  • 配列類似性
    Belongs to the zinc-containing alcohol dehydrogenase family.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ADH beta subunit antibody
    • ADH1B antibody
    • ADH1B_HUMAN antibody
    • ADH2 antibody
    • Alcohol dehydrogenase 1B antibody
    • alcohol dehydrogenase 2 (class I), beta polypeptide antibody
    • Alcohol dehydrogenase 2 antibody
    • Alcohol dehydrogenase subunit beta antibody
    • Aldehyde reductase antibody
    • DKFZp686C06125 antibody
    • OTTHUMP00000220192 antibody
    see all

画像

  • Anti-ADH1B antibody (ab175515) at 1/1000 dilution + HepG2 cell line lysate at 35 µg

    Secondary
    Anti-rabbit HRP conjugated antibody at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 40 kDa

  • Immunofluorescence analysis of HepG2 cells labeling ADH1B with ab175515 at a 1/10 dilution, followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

  • Immunohistochemical analysis of formalin fixed, paraffin-embedded Human hepatocarcinoma tissue labeling ADH1B with ab175515 at a 1/50 dilution, followed by DAB staining.

  • Flow cytometry analysis of HepG2 cells labeling ADH1B with ab175515 at 1/10 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

参考文献

ab175515 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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