Application
Western blot
Sample
Caenorhabditis elegans Tissue lysate - whole (whole organism)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
40 µg
Treatment
70c for 10 minutes equal parts sample and 2x Laemmli buffer
Specification
whole organism
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 23°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: antibody buffer - 0.1g milk + 50mL TTBS
Dilution
1/2000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Anti-rabbit IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 100 kDa Non-specific: 22, 60 kDa
Positive control
Tubulin
Additional data
Additional Notes
ADR-1 (C. elegans ADA homolog) was confirmed in band at 100kd by protein mass spec
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投稿 Mar 29 2018