Application
Western blot
Sample
Human Cell lysate - whole cell (HT1080 cells)
Loading amount
10 µg
Specification
HT1080 cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 5% Marvel TBS-T
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit HRP-linked
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
8 minute(s) and 0 second(s)
Bands
Specific: 75 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 02 2009