Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HT1080 Cell line)
Total protein in input
100000 cells
Specification
HT1080 Cell line
Treatment
Pulse labelled with 35S-Met for one hour
Immuno-precipitation step
Protein G
Other product details
Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: NDET
Western Blot antibody
Western Blot antibody
None used
Additional data
Additional Notes
8-16% gel impregnated with enhance. NDET is 1% NP40, 0.4% Deoxycholate, 66 mM EDTA, 10 mM Tris pH 7.4. Specific bands of approximately 100, 80 kDa. Band at ~50kDa is presumed non-specific.
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投稿 Jan 05 2009