Anti-ADAM12 抗体 (ab28747)
Key features and details
- Goat polyclonal to ADAM12
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ADAM12 antibody
ADAM12 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to ADAM12 -
由来種
Goat -
特異性
This antibody is expected to recognise both the longer membrane bound form of human ADAM12 and the shorter soluble ADAM12 splice isoform. This antibody does not cross react with other ADAMS. -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide:
AARPLPVSPARALC
, corresponding to N terminal amino acids 2-15 of ADAM12. -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab28747の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 100 kDa).
1 hour primary incubation is recommended for this product. |
|
IHC-P |
Use a concentration of 3.75 µg/ml.
|
特記事項 |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 100 kDa). 1 hour primary incubation is recommended for this product. |
IHC-P
Use a concentration of 3.75 µg/ml. |
ターゲット情報
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機能
Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors. -
組織特異性
Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas. -
配列類似性
Contains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain. -
ドメイン
The cysteine-rich domain supports cell adhesion through syndecans and triggers signaling events that lead to beta-1 integrin-dependent cell spreading. In carcinomas cells the binding of this domain to syndecans does not allow the integrin-mediated cell spreading.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻訳後修飾
The precursor is cleaved by a furin endopeptidase. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 8038 Human
- Omim: 602714 Human
- SwissProt: O43184 Human
- Unigene: 594537 Human
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別名
- A disintegrin and metalloproteinase domain 12 antibody
- ADA12_HUMAN antibody
- ADAM 12 antibody
see all
画像
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Anti-ADAM12 antibody (ab28747) at 0.1 µg/ml + Human heart lysate (RIPA buffer, 30µg total protein per lane)
Predicted band size: 100 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Primary incubation was for 1 hour.
Detected using chemiluminescence.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab28747 は 3 報の論文で使用されています。
- Chung MW et al. Expression of ADAM12 in Gastric Cancer and its Relation to Tumor Cell Behavior and Prognosis. In Vivo 36:2194-2204 (2022). PubMed: 36099146
- Liu J et al. MicroRNA-144 inhibits the metastasis of gastric cancer by targeting MET expression. J Exp Clin Cancer Res 34:35 (2015). WB . PubMed: 25927670
- Estrella C et al. Role of A disintegrin and metalloprotease-12 in neutrophil recruitment induced by airway epithelium. Am J Respir Cell Mol Biol 41:449-58 (2009). IHC-P ; Human . PubMed: 19213876