Anti-ACTH 抗体 [AH26] - BSA and Azide free (ab212734)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [AH26] to ACTH - BSA and Azide free
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-ACTH antibody [AH26] - BSA and Azide free
ACTH 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [AH26] to ACTH - BSA and Azide free -
由来種
Mouse -
特異性
ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This antibody is specific to Synacthen (aa 1-24 of ACTH); does not react with CLIP (aa 17-39 of ACTH).
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アプリケーション
適用あり: IHC-P, IHC-Frmore details
適用なし: WB -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Sheep, Guinea pig, Cow, a wide range of other species, Macaque monkey -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- IHC-P: Human, Mouse and Rat pituitary gland tissue. IHC-Fr: Mouse pituitary gland tissue
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特記事項
This product has switched from a hybridoma to recombinant production method on 11th March 2022.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
AH26 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab212734の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IHC-Fr |
Use at an assay dependent concentration.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IHC-Fr
Use at an assay dependent concentration. |
ターゲット情報
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関連性
ACTH occurs in cells of the anterior pituitary and in neurons in brain. It regulates the corticosteroid production in the adrenal cortex. Beta endorphin and Met enkephalin are endogenous opiates. MSH (melanocyte stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes. -
細胞内局在
Secreted -
参照データベース
- Entrez Gene: 5443 Human
- Entrez Gene: 694430 Macaque monkey
- Entrez Gene: 18976 Mouse
- Entrez Gene: 24664 Rat
- Entrez Gene: 443212 Sheep
- Omim: 176830 Human
- SwissProt: P01190 Cow
- SwissProt: P19402 Guinea pig
see all -
別名
- ACTH antibody
- Adrenocorticotropic hormone antibody
- Adrenocorticotropin antibody
see all
画像
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This data was developed using ab233953, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human pituitary gland tissue labeling ACTH with ab233953 at 1/5000 (0.233 µg/ml) dilution, followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Positive staining on human pituitary gland. The section was incubated with ab233953 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879)
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
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This data was developed using ab233953, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse pituitary gland tissue labeling ACTH with ab233953 at 1/10000 (0.116 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse pituitary gland. The section was incubated with ab233953 for 30 mins at room temperature and then combined with specific mouse IgG antibody (ab125913, 1:1000) for 8mins.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection)
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
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This data was developed using ab233953, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat pituitary gland tissue labeling ACTH with ab233953 at 1/10000 (0.116 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat pituitary gland. The section was incubated with ab233953 for 30 mins at room temperature and then combined with specific mouse IgG antibody (ab125913, 1:1000) for 8mins.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection)
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
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This data was developed using ab233953, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse pituitary (fresh frozen) tissue labeling ACTH with ab233953 at 1/100 (11.64 µg/ml) dilution followed by ab150113, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green).
Positive staining on mouse pituitary. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)
データシートおよび資料
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Datasheet download
参考文献 (0)
ab212734 は論文での使用が確認できていません。