Anti-ACTH 抗体 [57] - N-terminal (ab233954)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [57] to ACTH - N-terminal
- Suitable for: mIHC, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ACTH antibody [57] - N-terminal
ACTH 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [57] to ACTH - N-terminal -
由来種
Mouse -
アプリケーション
適用あり: mIHC, IHC-Pmore details
適用なし: Flow Cyt,ICC or WB -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human ACTH (N terminal) conjugated to keyhole limpet haemocyanin.
Database link: P01189 -
ポジティブ・コントロール
- IHC-P: Human, mouse and rat pituitary gland tissues. mIHC: Human pituitary gland.
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特記事項
This product was switched from a hybridoma to recombinant production format on 25th November 2021.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
57 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab233954の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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mIHC |
1/5000.
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IHC-P |
1/5000 - 1/20000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Recommend 1:5000 for human species and 1:20, 000 for mouse and rat species |
特記事項 |
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mIHC
1/5000. |
IHC-P
1/5000 - 1/20000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Recommend 1:5000 for human species and 1:20, 000 for mouse and rat species |
ターゲット情報
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関連性
ACTH occurs in cells of the anterior pituitary and in neurons in brain. It regulates the corticosteroid production in the adrenal cortex. Beta endorphin and Met enkephalin are endogenous opiates. MSH (melanocyte stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes. -
細胞内局在
Secreted -
参照データベース
- Entrez Gene: 5443 Human
- Entrez Gene: 18976 Mouse
- Entrez Gene: 24664 Rat
- Omim: 176830 Human
- SwissProt: P01189 Human
- SwissProt: P01193 Mouse
- SwissProt: P01194 Rat
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別名
- ACTH antibody
- Adrenocorticotropic hormone antibody
- Adrenocorticotropin antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human pituitary gland labelling ACTH with ab233954 at 1/5000 dilution (0.214 μg/ml) (B), Luteinizing Hormone beta with ab221494 at 1/4000 dilution (0.258 μg/ml) (C) and Growth Hormone with ab155276 at 1/5000 dilution (0.044 μg/ml) (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-Growth Hormone (gray; Opal™690), anti-ACTH (green; Opal™520) and anti-Luteinizing Hormone beta (red; Opal™570) on human pituitary gland.
Panel B: anti-ACTH stained on corticotrophs.
Panel C: anti-Luteinizing Hormone beta stained on gonadotrophs.
Panel D: anti-Growth Hormone stained on somatotrophs.The section was incubated in three rounds of staining: in the order of ab155276, ab233954, and ab221494 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
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Fluorescence multiplex immunohistochemical analysis of human pituitary gland tissue (formalin-fixed paraffin-embedded section). Panel A shows merged staining of anti-ACTH stained on corticotrophs (ab233954; green; Opal™520) at 1:5000 (0.214 μg/ml) [Panel B], anti-TSH beta stained on thyrotrophs (ab155958; red; Opal™570) at 1:500 (1.728 μg/ml) [Panel C], and anti-Growth Hormone stained on somatotrophs (ab155276; gray; Opal™690) at 1:5000 (0.044 μg/ml) [Panel D] on human pituitary gland. DAPI was used as a nuclear counter stain. Followed by Opal Polymer HRP Ms + Rb secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. The section was incubated in three rounds of staining: in the order of ab155276, ab233954, and ab155958 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.
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Immunohistochemical analysis of Human pituatary gland tissue stained for ACTH using ab233954 at 1/5000 dilution. Positive staining on human pituitary gland. The section was incubated with ab233954 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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Immunohistochemical analysis of Mouse pituatary gland tissue stained for ACTH using ab233954 at 1/20000 dilution. Positive staining on mouse pituitary gland. The section was incubated with ab233954 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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Immunohistochemical analysis of Rat pituatary gland tissue stained for ACTH using ab233954 at 1/20000 dilution. Positive staining on rat pituitary gland. The section was incubated with ab233954 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab233954 は論文での使用が確認できていません。