Anti-ABCB5 抗体 (ab77549)
Key features and details
- Goat polyclonal to ABCB5
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ABCB5 antibody
ABCB5 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to ABCB5 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Horse, Cow, Chimpanzee -
免疫原
Synthetic peptide:
C-QTQHRNTSKKAQ
, corresponding to internal sequence amino acids 460-471 of Human ABCB5 (NP_848654.3). -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab77549 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77549の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
1 hour primary incubation is recommended for this product. |
特記事項 |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Plasma membrane transporter able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Responsible for the resistance to doxorubicin of a subset of malignant melanomas. -
組織特異性
Expressed by CD133-expressing progenitor cells among epidermal melanocytes (at protein level). Widely expressed with specific expression in pigment cells. Also expressed in several malignant tissues. -
配列類似性
Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
Contains 1 ABC transmembrane type-1 domain.
Contains 2 ABC transporter domains. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 340273 Human
- Omim: 611785 Human
- SwissProt: Q2M3G0 Human
- Unigene: 404102 Human
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別名
- ABCB 5 antibody
- ABCB5 antibody
- ABCB5 P-gp antibody
see all
画像
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Anti-ABCB5 antibody (ab77549) at 0.03 µg/ml + A431 cell lysate (in RIPA buffer) at 35 µg
Predicted band size: 90 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. -
Anti-ABCB5 antibody (ab77549) at 0.3 µg/ml + Human skin Lysate in RIPA buffer. at 35 µg
Developed using the ECL technique.
Predicted band size: 90 kDa
Observed band size: 90 kDa
Primary incubation was 1 hour.
データシートおよび資料
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Datasheet download
参考文献 (6)
ab77549 は 6 報の論文で使用されています。
- Louphrasitthiphol P et al. ABCB5 is activated by MITF and ß-catenin and is associated with melanoma differentiation. Pigment Cell Melanoma Res 33:112-118 (2020). PubMed: 31595650
- Boulding T et al. LSD1 activation promotes inducible EMT programs and modulates the tumour microenvironment in breast cancer. Sci Rep 8:73 (2018). PubMed: 29311580
- Keniya MV et al. Drug resistance is conferred on the model yeast Saccharomyces cerevisiae by expression of full-length melanoma-associated human ATP-binding cassette transporter ABCB5. Mol Pharm 11:3452-62 (2014). PubMed: 25115303
- Lai CY et al. CD133+ melanoma subpopulations contribute to perivascular niche morphogenesis and tumorigenicity through vasculogenic mimicry. Cancer Res 72:5111-8 (2012). IHC-P ; Human . PubMed: 22865455
- Kupas V et al. RANK is expressed in metastatic melanoma and highly upregulated on melanoma-initiating cells. J Invest Dermatol 131:944-55 (2011). Flow Cyt ; Human . PubMed: 21270824
- Cheung ST et al. Granulin-epithelin precursor and ATP-dependent binding cassette (ABC)B5 regulate liver cancer cell chemoresistance. Gastroenterology 140:344-55 (2011). Flow Cyt ; Human . PubMed: 20682318