Anti-A1CF/ACF 抗体 (ab89050)
Key features and details
- Mouse polyclonal to A1CF/ACF
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-A1CF/ACF antibody
A1CF/ACF 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to A1CF/ACF -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human A1CF/ACF aa 1-600. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_620311.1 -
ポジティブ・コントロール
- Placenta or HeLa whole cell lysate (ab150035).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab89050の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa. |
ターゲット情報
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機能
Essential component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in APOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit, APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also protects the edited APOB mRNA from nonsense-mediated decay. -
組織特異性
Widely expressed with highest levels in brain, liver, pancreas, colon and spleen. -
配列類似性
Contains 3 RRM (RNA recognition motif) domains. -
ドメイン
The RRM domains are necessary but not sufficient for binding to APOB mRNA. Additional residues in the pre-RRM and C-terminal regions are required for RNA-binding and for complementing APOBEC1 activity. -
細胞内局在
Nucleus. Endoplasmic reticulum. Cytoplasm. Predominantly nuclear where it localizes to heterochromatin. Also cytoplasmic where it is found at the outer surface of the endoplasmic reticulum (By similarity). Shuttles between the nucleus and cytoplasm. May be transported into the nucleus by the nuclear import protein TNPO2/TRN2 or by APOBEC1. - Information by UniProt
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参照データベース
- Entrez Gene: 29974 Human
- SwissProt: Q9NQ94 Human
- Unigene: 282795 Human
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別名
- A1CF antibody
- A1CF_HUMAN antibody
- ACF antibody
see all
画像
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Anti-A1CF/ACF antibody (ab89050) at 1 µg/ml + Human placenta lysate at 50 µg
Predicted band size: 65 kDa -
Anti-A1CF/ACF antibody (ab89050) at 1 µg/ml + HeLa cell lysate at 50 µg
Predicted band size: 65 kDa -
All lanes : Anti-A1CF/ACF antibody (ab89050) at 1 µg/ml
Lane 1 : A1CF/ACF-transfected 293T cell lysate
Lane 2 : non transfected lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 65 kDa
Observed band size: 65 kDa
データシートおよび資料
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Datasheet download
参考文献 (2)
ab89050 は 2 報の論文で使用されています。
- Liu Q et al. High APOBEC1 Complementation Factor Expression Positively Modulates the Proliferation, Invasion, and Migration of Endometrial Cancer Cells Through Regulating P53/P21 Signaling Pathway. Cancer Biother Radiopharm N/A:N/A (2020). PubMed: 32818382
- Fossat N et al. C to U RNA editing mediated by APOBEC1 requires RNA-binding protein RBM47. EMBO Rep : (2014). PubMed: 24916387