• 製品名Anti-8 Hydroxyguanosine antibody
    8 Hydroxyguanosine 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to 8 Hydroxyguanosine
  • 特異性ab10802 cross reacts completely with 8-OHdG,and does not cross react with other naturally occurring nucleotides.
  • アプリケーション適用あり: IHC-P, ELISA, ICC/IFmore details
  • 種交差性
    Due to the conserved nature of the target reactivity would be predicted in wide range of species.
  • 免疫原

    8-Hydroxyguanosine - conjugate.

  • ポジティブ・コントロール
    • Stressed tissue such as Alzheimer's affected neurons. or Human brain pre treated with 10ug proteinase K for 40mins at 37oC


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: Whole Serum
  • 精製度Whole antiserum
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野


Our Abpromise guarantee covers the use of ab10802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200.

Perform enzymatic antigen retrieval with 10 µg/ml proteinase K for 40 minutes at 37°C.

ELISA 1/100000 - 1/250000.
ICC/IF Use at an assay dependent concentration. PubMed: 16678792


  • 関連性8-Hydroxydeoxyguanosine (8OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging, and neurodegenerative conditions, such as Alzheimer’s disease. 8OHdG has become increasing popular as a sensitive, stable and integral marker of oxidative damage in cellular DNA. Biomonitoring in humans has demonstrated that 8OHdG can be excreted in the urine, and that a significant increase is caused by exposure to tobacco smoke and ionizing radiation. Because 8OHdG is so well correlated with oxidative stress and damage to DNA, which leads to degenerative disease states, the development of an antibody that can be used to study DNA damage has numerous applications. In addition to the direct study of DNA damage within cells, this antibody has applications in the development of immunoassays that can monitor 8OHdG excretion in the urine and serve as a biomarker of oxidative stress.
  • 別名
    • 8 hydroxy 2’ deoxyguanosine antibody
    • 8 hydroxyguanine antibody
    • 8 hydroxyguanosine antibody
    • 8 OHG antibody
    • 8-OHG antibody
    • 8OG antibody
    • 8OHdG antibody
    • 8OHG antibody
    see all

Anti-8 Hydroxyguanosine antibody 画像

  • Immunohistochemical analysis of cat hepatic tissue, labeling 8 Hydroxyguanosine with ab10802. Sample fixed in paraformaldehyde. Heat mediated antigen retrieval with Tris-EDTA (pH9). Treated with ab10802 diluted 1/1500 in PBS plus casein for 1 hour and 30 minutes at 37°C. Secondary was ImmPress anti-goat HRP-conjugated polyclonal antibody.

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  • IHC staining on Alzheimer disease brain showing oxidized RNA in neurons. The tissue sections were formalin-fixed, paraffin embedded with either (A) no pretreatment or (B) pretreatment with 10 µg/ml proteinase K for 40 minutes at 37°C. Each was stained with a 1:200 dilution.

Anti-8 Hydroxyguanosine antibody (ab10802) 使用論文

This product has been referenced in:
  • Johansen AK  et al. The serotonin transporter promotes a pathological estrogen metabolic pathway in pulmonary hypertension via cytochrome P450 1B1. Pulm Circ 6:82-92 (2016). IHC-P ; Mouse . Read more (PubMed: 27162617) »
  • Zhang G  et al. The anti-oxidative role of Micro-vesicles derived from human Wharton-Jelly mesenchymal stromal cells through NOX2/gp91(phox) suppression in alleviating renal ischemia-reperfusion injury in rats. PLoS One 9:e92129 (2014). IHC-P ; Rat . Read more (PubMed: 24637475) »

See all 8 Publications for this product

Product Wall

This product has not been purified and is sold as a whole antiserum therefore we have not determined its concentration. As a rough guideline, in general whole antiserum has a concentration of 1-10 mg/ml.

The antibody will detect 8-OHdG in DNA and also RNA. RNase digestion recommended if you want to detect only 8-OHdG in DNA.

The paper at the following link used a different antibody, a mouse monoclonal antibody, but the procedure for staining ...

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The immunostain that used the antibody at 1/4000 used a biotin-conjugated secondary, so it is likely that this was followed with an avidin-biotin-HRP complex amplification. It is possible that 1/4000 was in fact effective, as antibody affinities vary a...

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Are you confident the secondary antibody and other reagents are effective? If so, I agree that it is best to try a different antibody at this point, which I will send free-of-charge.

Abcam has several others against 8-Hydroxyguanosine but as...

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Thank you for your enquiry.

I can confirm that ab10802 Anti-8 Hydroxyguanosine antibody is tested as suitable for indirect ELISA.

However, I am sorry to confirm that as far as we are aware, ab10802 has never been tested in flow cy...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cat Tissue sections (liver)
Specification liver
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA (pH 9.0)
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C

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Verified customer

投稿 Feb 04 2013

Thank you for contacting us.

My Spanish speaking colleague is currently out of the office, unfortunately my Spanish is not good enough to write my reply in your language.

As stated on the product datasheets, both anti-8 Hydroxyguanosi...

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I appreciate the time taken to submit further information to us.

Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functioning in the applicatio...

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I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

In or...

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Wie am Telefon versprochen, sende ich Ihnen hier den Link zu der Publikation, von welcher das Protokoll als Vorbild genommen wurde:


Die Autoren der Publikation habeneinenmonoklonalen Antik...

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