Anti-70 kD Neurofilament Light 抗体 [EPR2459(2)] (ab108363)

製品の概要

  • 製品名Anti-70 kD Neurofilament Light antibody [EPR2459(2)]
    70 kD Neurofilament Light 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2459(2)] to 70 kD Neurofilament Light
  • アプリケーション適用あり: WB, IPmore details
    適用なし: Flow Cyt,ICC/IF or IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human 70 kD Neurofilament Light (internal sequence).

  • ポジティブ・コントロール
    • C6, fetal brain and HeLa cell lysates
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000. Detects a band of approximately 68 kDa (predicted molecular weight: 62 kDa).
IP 1/110.
  • 追加情報Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • ターゲット情報

    • 機能Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
    • 関連疾患Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 1F (CMT1F) [MIM:607734]. CMT1F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT1 group are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. CMT1F is characterized by onset in infancy or childhood (range 1 to 13 years).
      Defects in NEFL are the cause of Charcot-Marie-Tooth disease type 2E (CMT2E) [MIM:607684]. CMT2E is an autosomal dominant form of Charcot-Marie-Tooth disease type 2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy.
    • 配列類似性Belongs to the intermediate filament family.
    • ドメインThe extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.
    • 翻訳後修飾O-glycosylated.
      Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 68 kDa neurofilament protein antibody
      • CMT1F antibody
      • CMT2E antibody
      • NEFL antibody
      • Neurofilament light polypeptide 68kDa antibody
      • Neurofilament light polypeptide antibody
      • Neurofilament protein light chain antibody
      • Neurofilament protein light polypeptide antibody
      • Neurofilament triplet L protein antibody
      • NF L antibody
      • NF-L antibody
      • NF68 antibody
      • NFL antibody
      • NFL_HUMAN antibody
      see all

    Anti-70 kD Neurofilament Light antibody [EPR2459(2)] 画像

    • Anti-70 kD Neurofilament Light antibody [EPR2459(2)] (ab108363) at 1/5000 dilution (purified) + C6 cell lysate at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 62 kDa
      Observed band size : 68 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-70 kD Neurofilament Light antibody [EPR2459(2)] (ab108363) at 1/5000 dilution (purified) + Mouse brain tissue lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 62 kDa
      Observed band size : 68 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-70 kD Neurofilament Light antibody [EPR2459(2)] (ab108363) at 1/5000 dilution (purified) + SH-SY5Y cell lysate at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 62 kDa
      Observed band size : 68 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-70 kD Neurofilament Light antibody [EPR2459(2)] (ab108363) at 1/1000 dilution (purified) + Human fetal brain tissue lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 62 kDa
      Observed band size : 68 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • ab108363 (purified) at 1/110 immunoprecipitating 70 kDa Neurofilament Light in human fetal brain tissue lysate (Lane 1). For western blotting, a HRP-conjugated anti-rabbit IgG was used as the secondary antibody (1/1000).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-70 kD Neurofilament Light antibody [EPR2459(2)] (ab108363) at 1/1000 dilution (unpurified)

      Lane 1 : C6 cell lysate
      Lane 2 : Human Fetal brain cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 62 kDa
      Observed band size : 68 kDa (why is the actual band size different from the predicted?)

    Anti-70 kD Neurofilament Light antibody [EPR2459(2)] (ab108363) 使用論文

    ab108363 has not yet been referenced specifically in any publications.

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