製品の概要

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, pH 7
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to horseradish peroxidase.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/5000 - 1/20000.
WB 1/1000 - 1/5000.

ターゲット情報

  • 関連性The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 細胞内局在Depends upon the localization of the parent protein tagged with hexahistidine.
  • 別名
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody (HRP) 画像

  • All lanes : Anti-6X His tag® antibody (HRP) (ab1187) at 1/4000 dilution

    Lane 1 : 4ug Human His-tagged protein purified from E.coli
    Lane 2 : 2ug Human His-tagged protein purified from E.coli
    Lane 3 : 1ug Human His-tagged protein purified from E.coli
    Lane 4 : 0.5ug Human His-tagged protein purified from E.coli


    Observed band size : 136 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an Abreview submitted by Mr Ohrt Thomas

    See Abreview

Anti-6X His tag® antibody (HRP) (ab1187) 使用論文

This product has been referenced in:
  • Schulte T  et al. The BR domain of PsrP interacts with extracellular DNA to promote bacterial aggregation; structural insights into pneumococcal biofilm formation. Sci Rep 6:32371 (2016). WB . Read more (PubMed: 27582320) »
  • Nkolola JP  et al. Characterization and immunogenicity of a novel mosaic M HIV-1 gp140 trimer. J Virol 88:9538-52 (2014). ELISA . Read more (PubMed: 24965452) »

See all 30 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (mouse pancreatic cancer primary cell lines)
Loading amount 25 µg
Specification mouse pancreatic cancer primary cell lines
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Abcam user community

Verified customer

投稿 Feb 09 2011

Western blot

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 0.1 µg
Gel Running Conditions Reduced Denaturing
Sample Human Recombinant protein (purification sur colonne HIS Trap)
Specification purification sur colonne HIS Trap
Blocking step caséine as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 May 30 2013

Thank you for calling Abcam earlier. After having spoken to the lab, I would advise you not to store ab1187 at at -20C as it will cause the product to lose some functionality due to the HRP conjugation.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Purified protein (purified His-tagged lipase, SPINK1)
Loading amount 1 µg
Specification purified His-tagged lipase, SPINK1
Gel Running Conditions Reduced Denaturing (15%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Miklos Sahin-Toth

Verified customer

投稿 Aug 12 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (neuronal cell)
Loading amount 20 µg
Specification neuronal cell
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Feb 09 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Purified protein (Purified His-tagged extracellular human LDLR)
Loading amount 0.1 µg
Specification Purified His-tagged extracellular human LDLR
Gel Running Conditions Reduced Denaturing (4-20% gel)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Aug 25 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Escherichia coli Purified protein (BL21(DE3))
Specification BL21(DE3)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Type Sandwich (Capture)
Username

Abcam user community

Verified customer

投稿 Jan 27 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Escherichia coli Purified protein (BL21(DE3))
Loading amount 0.01 µg
Specification BL21(DE3)
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Dec 30 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Purified protein (recombinant human fibronectin domain expressed in)
Loading amount 1 µg
Specification recombinant human fibronectin domain expressed in
Gel Running Conditions Reduced Denaturing (12% BisTris NuPAGE gel)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Sep 09 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Rat Serum (injected his-yeagged peptide)
Specification injected his-yeagged peptide
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Type Sandwich (Detection)
Username

Dr. Sandra Reichstetter

Verified customer

投稿 Dec 11 2007

1-10 of 16 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"