Anti-6X His tag® 抗体 [HIS-1] (Alkaline Phosphatase) (ab49746)

製品の概要

  • 製品名Anti-6X His tag® antibody [HIS-1] (Alkaline Phosphatase)
    6X His tag® 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [HIS-1] to 6X His tag® (Alkaline Phosphatase)
  • 標識Alkaline Phosphatase
  • 特異性ab49746 recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors. The product is reactive with fusion protein expressed by prokaryotic pET, pRSET and pTrc expression vectors.
  • アプリケーション適用あり: WB, Dot blot, ELISAmore details
  • 免疫原

    Recombinant poly(Histidine)-tagged fusion protein.

  • ポジティブ・コントロール
    • Bacteria lysates expressing a recombinant polyHistidine tagged fusion protein and BCIP/NBT substrate.
  • 特記事項

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

    Monoclonal antibodies reacting specifically with poly-histidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidinetagged fusion protein expressing vectors.

    The antibody is prepared by conjugation of calf intestinal alkaline phosphatase to mouse monoclonal anti-polyHistidine purified from ascites fluid of the HIS-1 hybridoma

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: 50% Glycerol, 1% BSA, 0.05M Tris buffer, 1mM Magnesium chloride, pH 8.0
  • Concentration information loading...
  • 特記事項(精製)Immunoglobulin fraction purified from ascites fluid.
  • 一次抗体 備考Monoclonal antibodies reacting specifically with poly-histidine may be useful in various immunotechniques, to identify the expression of a polyhistidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a polyHistidinetagged fusion protein expressing vectors.
  • ポリ/モノモノクローナル
  • クローン名HIS-1
  • アイソタイプIgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab49746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Predicted molecular weight: 7 kDa.
Dot blot Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 関連性The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 細胞内局在Depends upon the localization of the parent protein tagged with hexahistidine.
  • 別名
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody [HIS-1] (Alkaline Phosphatase) (ab49746) 使用論文

This product has been referenced in:
  • Henche AL  et al. Dissection of key determinants of cleavage activity in signal peptidase III (SPaseIII) PibD. Extremophiles 18:905-13 (2014). Read more (PubMed: 25102813) »
  • Hussack G  et al. Neutralization of clostridium difficile toxin A with single-domain antibodies targeting the cell-receptor binding domain. J Biol Chem : (2011). WB . Read more (PubMed: 21216961) »

See all 2 Publications for this product

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Thank you for contacting Abcam.

The 6x His-tag should essentially be the same no matter which vector it is expressed by. The affinity of the antibody is more likely to be affected by the proximity of the His-tag to the recombinant protein (N...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (neuronal cell)
Loading amount 10 µg
Specification neuronal cell
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Dec 02 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"