Anti-6X His tag® 抗体 [HIS-1] (ab49936)

製品の概要

  • 製品名
    Anti-6X His tag® antibody [HIS-1]
    6X His tag® 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [HIS-1] to 6X His tag®
  • アプリケーション
    適用あり: Flow Cyt, WB, Dot blot, ELISA, ICC/IFmore details
  • 免疫原

    Recombinant full length protein corresponding to 6X His tag®.

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016 - GR224242. Lot numbers higher than GR224242 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    pH: 7.4
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度
    Proprietary Purification
  • 特記事項(精製)
    Purified from Tissue culture supernatant.
  • ポリ/モノ
    モノクローナル
  • クローン名
    HIS-1
  • アイソタイプ
    IgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab49936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration.
WB 1/3000.
Dot blot Use at an assay dependent concentration.

Use at an assay dependent concentration.

ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 細胞内局在
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • 別名
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody [HIS-1] (ab49936) 使用論文

ab49936 has not yet been referenced specifically in any publications.

Product Wall

Using 50ul of diluted antibody per well is an adequate amount although this amount may be optimized in your experiments. As this product is an unpurified ascites fluid, I would recommend using a slightly higher concentration of this product closer to 1...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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