Anti-6X His tag® 抗体 (FITC) (ab3554)

製品の概要

  • 製品名
    Anti-6X His tag® antibody (FITC)
    6X His tag® 一次抗体 製品一覧
  • 製品の詳細
    Chicken polyclonal to 6X His tag® (FITC)
  • 標識
    FITC. Ex: 493nm, Em: 528nm
  • アプリケーション
    適用あり: WB, ELISA, IHC-Frmore details
  • 免疫原

    Synthetic peptide: HHHHHH conjugated to KLH.

  • 特記事項

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

    Molar F/P ratio is 3.6:1.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    PBS, 0.2% BSA, 0.1% sodium azide, pH7.0
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • ミエローマ
    unknown
  • アイソタイプ
    IgY
  • 軽鎖の種類
    unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab3554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration.

Prior to conjugation, the antibodies were analyzed by Western Blot.

ELISA Use at an assay dependent concentration.

Prior to conjugation, the antibodies were analyzed by ELISA.

IHC-Fr 1/100.

ターゲット情報

  • 関連性
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 細胞内局在
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • 別名
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody (FITC) 画像

  • ab3554 staining 6X His tag® in rat spinal cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with TritonX and blocked with 6% serum for 2 hours at 21°C. Samples were incubated with primary antibody (1/100 in 0.1M PBS + 0.3% Triton + 6% NGS) for 17 hours at 4°C.

    See Abreview

Anti-6X His tag® antibody (FITC) (ab3554) 使用論文

ab3554 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 21°C
Sample
Rat Tissue sections (spinal cord tissue)
Specification
spinal cord tissue
Permeabilization
Yes - TritonX
Fixative
Paraformaldehyde
Username

Dr. Hayk Petrosyan

Verified customer

投稿 Jun 23 2014

I cannot advise performing western blotting with a FITC antibody although it is likely to work given a suitable secondary antibody that recognizes the primary chicken antiserum. The reason behind the fact that western blotting is a listed application i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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