Anti-6X His tag® 抗体 (FITC) (ab1270)

製品の概要

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPBS with 0.2% BSA, 0.1% sodium azide
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC 1/200 - 1/2000.

ターゲット情報

  • 関連性The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 細胞内局在Depends upon the localization of the parent protein tagged with hexahistidine.
  • 別名
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody (FITC) (ab1270) 使用論文

ab1270 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your email and I'm sorry to hear that you are experiencing difficulty with ab1270. Can you please tell me the lot number that you received (it is indicated on the vial)? Did you order directly from Abcam or through one of our distributors...

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Thank you for your enquiry. I regret to inform you that ab1270 has not yet been tested in IHC, only in ICC. There was an error on the datasheet which I have since updated. I apologize for this error and if you have any more questions, please contact us...

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The antibody is designed to react with 6 His at the N-terminus and C-terminus.

The method of fixation is unlikely to have significant effect on the His-tag. However, the fixation may influence the conformation of the tagged protein, and thereby, affect the accessibility of the His-tag. Thus, one must empirically determine the i...

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Cross-reactivities have not yet been reported, but this antibody should be specific for the 6x-His epitope tag.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"