製品の概要

  • 製品名Anti-6X His tag® antibody (Biotin)
    6X His tag® 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to 6X His tag® (Biotin)
  • 標識Biotin
  • 特異性This antibody recognises 6x His tag.
  • アプリケーション適用あり: Flow Cyt, WB, ELISAmore details
  • 免疫原

    HHHHHH conjugated with KLH.

  • 特記事項HIS-TAG® is a trademark of EMD Biosciences, Inc.

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.2% BSA, PBS, pH 7.2
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)This antibody was purified using the peptide immobilized on a solid support.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab27025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration.

ab200208 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/1000 - 1/5000.
ELISA 1/2000 - 1/10000.

ターゲット情報

  • 関連性The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 細胞内局在Depends upon the localization of the parent protein tagged with hexahistidine.
  • 別名
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody (Biotin) (ab27025) 使用論文

This product has been referenced in:
  • Spång HC  et al. Heterodimeric Barnase-Barstar Vaccine Molecules: Influence of One versus Two Targeting Units Specific for Antigen Presenting Cells. PLoS One 7:e45393 (2012). WB . Read more (PubMed: 23028981) »
  • Lee HK  et al. In vivo requirement for Atg5 in antigen presentation by dendritic cells. Immunity 32:227-39 (2010). Read more (PubMed: 20171125) »

See all 2 Publications for this product

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not arrive with the volume stated on the datasheet and for the inconvenience ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Escherichia coli Purified protein (recombinant human fibronectin domain)
Loading amount 0.1 µg
Specification recombinant human fibronectin domain
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

投稿 Sep 04 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Saccharomyces cerevisiae Cell (yeast expressing a his tagged scFv)
Specification yeast expressing a his tagged scFv
Preparation Cell harvesting/tissue preparation method: yeast washed once in PBS
Sample buffer: PBS
Fixation none
Permeabilization No
Gating Strategy sample was gated to include all yeast cells
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Abcam user community

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投稿 Jun 24 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (Samples pre and post enzymatic his tag cleavage)
Loading amount 4 µg
Specification Samples pre and post enzymatic his tag cleavage
Treatment His tag purified supernatant followed by a reaction to cleave the his tag
Gel Running Conditions Non-reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Abcam user community

Verified customer

投稿 Jun 04 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"