製品の概要

  • 製品名
    Anti-6X His tag® antibody (Biotin)
    6X His tag® 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to 6X His tag® (Biotin)
  • 標識
    Biotin
  • 特異性
    This antibody recognises 6x His tag.
  • アプリケーション
    適用あり: Flow Cyt, WB, ELISAmore details
  • 免疫原

    HHHHHH conjugated with KLH.

  • 特記事項
    HIS-TAG® is a trademark of EMD Biosciences, Inc.

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.2% BSA, PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    This antibody was purified using the peptide immobilized on a solid support.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab27025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration.

ab200208 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/1000 - 1/5000.
ELISA 1/2000 - 1/10000.

ターゲット情報

  • 関連性
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 細胞内局在
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • 別名
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody (Biotin) (ab27025) 使用論文

This product has been referenced in:
  • Spång HC  et al. Heterodimeric Barnase-Barstar Vaccine Molecules: Influence of One versus Two Targeting Units Specific for Antigen Presenting Cells. PLoS One 7:e45393 (2012). WB . Read more (PubMed: 23028981) »
  • Lee HK  et al. In vivo requirement for Atg5 in antigen presentation by dendritic cells. Immunity 32:227-39 (2010). Read more (PubMed: 20171125) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Escherichia coli Purified protein (recombinant human fibronectin domain)
Loading amount
0.1 µg
Specification
recombinant human fibronectin domain
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

投稿 Sep 04 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Saccharomyces cerevisiae Cell (yeast expressing a his tagged scFv)
Specification
yeast expressing a his tagged scFv
Preparation
Cell harvesting/tissue preparation method: yeast washed once in PBS
Sample buffer: PBS
Fixation
none
Permeabilization
No
Gating Strategy
sample was gated to include all yeast cells
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Abcam user community

Verified customer

投稿 Jun 24 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Recombinant protein (Samples pre and post enzymatic his tag cleavage)
Loading amount
4 µg
Specification
Samples pre and post enzymatic his tag cleavage
Treatment
His tag purified supernatant followed by a reaction to cleave the his tag
Gel Running Conditions
Non-reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Jun 04 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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