Anti-6X His tag® 抗体 [AD1.1.10] (Phycoerythrin) (ab72467)

製品の概要

  • 製品名Anti-6X His tag® antibody [AD1.1.10] (Phycoerythrin)
    6X His tag® 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AD1.1.10] to 6X His tag® (Phycoerythrin)
  • 標識Phycoerythrin. Ex: 488nm, Em: 575nm
  • アプリケーション適用あり: Flow Cytmore details
  • 免疫原

    Synthetic peptide HHHHHH tag - tagged

  • 特記事項

    Excitation max. lambda: 565>498 nm Emission max. lambda: 578 nm F/P 1:1.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 2mM Sodium Azide
    Constituents: 0.1% BSA, 50mM Sucrose, 150mM Sodium chloride, 100mM Sodium phosphate, pH 7.4
  • Concentration information loading...
  • 精製度Size exclusion
  • 特記事項(精製)Size exclusion chromatography as well as some proprietary methods for purifying the final materials were used.
  • ポリ/モノモノクローナル
  • クローン名AD1.1.10
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab72467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/50 - 1/100. ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 関連性The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 細胞内局在Depends upon the localization of the parent protein tagged with hexahistidine.
  • 別名
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody [AD1.1.10] (Phycoerythrin) 画像

  • BALB-3T3 cells, which express moderate amounts of EGFR, were incubated with a His6-tagged anti-EGFR fibronectin domain. The labeled cells were washed and incubated with 50 microliters of 1:15 diluted PE-conjugated anti-His6 antibody. Cells were washed and analyzed by flow cytometry. A control without fibronectin domain was also analyzed. Image: Dashed black line is control without fibronectin. Solid blue line is sample with His6-tagged anti-EGFR fibronectin domain.

    See Abreview

Anti-6X His tag® antibody [AD1.1.10] (Phycoerythrin) (ab72467) 使用論文

ab72467 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. Indeed we have only tested this antibody for Flow Cytometry. You may wish to try an ELISA to test your solution, we have a protocol for ELISA with a fluorophore conjugated primary antibody here:

http://www.abcam.co...

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Thank you for contacting us and your interest in our products.
In answer to your questions:
1. Ab concentration of anti-6X His tag (PE), ab72467
The concentration of this antibody is 69 ug/mL.
2. The F:P ratio of the antibody
T...

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Thank you for your inquriy.

I am happy to confirm that we offer a isotype control for ab72467 that is also PE conjugated:

ab91357

http://www.abcam.com/index.html?datasheet=91357 (or use the following: http://www.abcam.com/...

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Thank you for contacting us.

The molecular weight of the antibody itsself will be ˜150 kDa plus anadditional 240 kDa from the PE fluorophore. The molecular weight detected by the antibody will depend on what protein is His-tagged. The 6X His ...

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Thank you for your reply.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of a new lot of ab72467.

To check the...

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Thank you for contacting us.

Your credit note ID is xxxx.

I am sorry that this antibody did not perform as stated on the datasheet. Your data clearly shows this products emission outside the range for a PE only conjugation. I have...

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Thanks for your call today and for letting us know about the problem with this antibody. As we discussed, I am sending a free of charge vial from a new lot on the order ***. We do have this in stock and it should arrive tomorrow. I apol...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Mutiple Myeloma)
Specification Mutiple Myeloma
Fixation no fixation
Permeabilization No
Gating Strategy one cell population
Username

Mr. Etienne Matous

Verified customer

投稿 Nov 17 2011

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has ca...

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Thank you for contacting us. After checking the stock of this antibody again; I can assure you that the antibody liquid should be light pink in colour. It may be possible that you might have received a bad vial of this antibody. I can certainly rep...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"