Anti-6X His tag® 抗体 [AD1.1.10] (DyLight® 650) (ab117504)

製品の概要

  • 製品名Anti-6X His tag® antibody [AD1.1.10] (DyLight® 650)
    6X His tag® 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AD1.1.10] to 6X His tag® (DyLight® 650)
  • 標識DyLight® 650. Ex: 654nm, Em: 673nm
  • アプリケーション適用あり: ICC/IF, Flow Cytmore details
  • 免疫原

    Synthetic peptide corresonding to 6X His tag®.

  • 特記事項Stable for 1yr.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーpH: 8.20
    Constituent: 99% Tris buffered saline
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名AD1.1.10
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab117504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/500 - 1/5000.
Flow Cyt Use at an assay dependent concentration.

ターゲット情報

  • 関連性The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 細胞内局在Depends upon the localization of the parent protein tagged with hexahistidine.
  • 別名
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody [AD1.1.10] (DyLight® 650) (ab117504) 使用論文

ab117504 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cells transfected with His-tagged actin)
Permeabilization Yes - 0.1% Triton-X100 in PBS
Specification HeLa cells transfected with His-tagged actin
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative Paraformaldehyde
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投稿 Oct 28 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"