Anti-6X His tag® 抗体 [AD1.1.10] (DyLight® 650) (ab117504)

製品の概要

  • 製品名
    Anti-6X His tag® antibody [AD1.1.10] (DyLight® 650)
    6X His tag® 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AD1.1.10] to 6X His tag® (DyLight® 650)
  • 標識
    DyLight® 650. Ex: 654nm, Em: 673nm
  • アプリケーション
    適用あり: ICC/IF, Flow Cytmore details
  • 免疫原

    Synthetic peptide corresonding to 6X His tag®.

  • 特記事項
    Stable for 1yr.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    pH: 8.20
    Constituent: 99% Tris buffered saline
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    AD1.1.10
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab117504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/500 - 1/5000.
Flow Cyt Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 細胞内局在
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • 別名
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody [AD1.1.10] (DyLight® 650) 画像

  • Immunocytochemistry/ Immunofluorescence analysis of HeLa cells transfected with His-tagged actin labeling 6X His tag® with ab117504 at 1/1000 dilution. The cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton-X100 in PBS. The cells were blocked with 5% BSA for 12 hours at 4°C, followed by incubation with Anti-6X His tag® antibody [AD1.1.10] (DyLight® 650) (ab117504) for 2 hours at 25°C.

    See Abreview

Anti-6X His tag® antibody [AD1.1.10] (DyLight® 650) (ab117504) 使用論文

This product has been referenced in:
  • Imhof BA  et al. CCN1/CYR61-mediated meticulous patrolling by Ly6Clow monocytes fuels vascular inflammation. Proc Natl Acad Sci U S A 113:E4847-56 (2016). Read more (PubMed: 27482114) »
  • Tillotson BJ  et al. Engineering an Anti-Transferrin Receptor ScFv for pH-Sensitive Binding Leads to Increased Intracellular Accumulation. PLoS One 10:e0145820 (2015). Read more (PubMed: 26713870) »

See all 2 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cells transfected with His-tagged actin)
Permeabilization
Yes - 0.1% Triton-X100 in PBS
Specification
HeLa cells transfected with His-tagged actin
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
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投稿 Oct 28 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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