Anti-6X His tag® 抗体 [AD1.1.10] (Biotin) (ab15145)

製品の概要

  • 製品名
    Anti-6X His tag® antibody [AD1.1.10] (Biotin)
    6X His tag® 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AD1.1.10] to 6X His tag® (Biotin)
  • 標識
    Biotin
  • 特異性
    The clone number has been updated from (3H2201) to (AD1.1.10) both clone numbers name the same antibody clone.
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    In Western Blot of bacterial extracts, the antibody has been shown not to crossreact with any endogenous products. Some Reactivity may be seen with extracts of insect or mammalian cells.
  • 免疫原

    His-His-His-His-His-His conjugated to KLH.

  • 特記事項
    HIS-TAG® is a trademark of EMD Biosciences, Inc.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    AD1.1.10
  • ミエローマ
    NS1
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab15145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000.
ELISA 1/500 - 1/1000.

ターゲット情報

  • 関連性
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 細胞内局在
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • 別名
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

プロトコール

Anti-6X His tag® antibody [AD1.1.10] (Biotin) (ab15145) 使用論文

ab15145 has not yet been referenced specifically in any publications.

Product Wall

The biotin binding of ab15145 has not been quantified, but it is typically in the range of 4-6 biotin molecules / antibody. The biotin is linked through lysine residues on the antibody surface.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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