製品の概要

  • 製品名
    Anti-6X His tag® antibody [AD1.1.10]
    6X His tag® 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AD1.1.10] to 6X His tag®
  • アプリケーション
    適用あり: ICC/IF, IHC-Fr, IP, WBmore details
  • 種交差性
    It reacts against all His tagged proteins so far tested. In Western blotting of bacterial extracts the antibody has been shown not to react with any endogenous products, although some Reactivity may be seen with extracts of insect or mammalian cells.
  • 免疫原

    PAX6 transcription factor linked to histidine tag.

  • 特記事項

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

     

    ab15149 recognises proteins containing the motif HHHHHH and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells.

    The clone number has been updated from (3H2201) to (AD1.1.10) both clone numbers name the same antibody clone.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    Tissue cuture supernatant purified by Protein G affinity chromatography.
  • 一次抗体 備考
    ab15149 recognises proteins containing the motif HHHHHH and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells.
  • ポリ/モノ
    モノクローナル
  • クローン名
    AD1.1.10
  • ミエローマ
    NS1
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab15149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/500 - 1/100.

ターゲット情報

  • 関連性
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 細胞内局在
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • 別名
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

参考文献

This product has been referenced in:

See all 5 Publications for this product

レビューと Q&A

I can confirm that all four products, the ab5000, ab15149, ab18184 and ab81663 detect proteins, which contain either an N or C-terminal HHHHHHGS (6x) epitope tag.

Important aspects for his tag recognition are, the localisation of the tag (N- o...

Read More

This antibody recognises proteins containing the motif HHHHHH (5x His) and it is therefore of value in detecting proteins containing histidine tags (either at the N- or C-terminal). The antibody may be used to track the purification of proteins express...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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