製品の概要

  • 製品名
    Anti-6X His tag® antibody
    6X His tag® 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to 6X His tag®
  • 特異性
    This antibody was verified by ELISA against peptide conjugated to BSA(HHHHHH/BSA).
  • アプリケーション
    適用あり: ELISA, ICC/IF, ICC, WBmore details
  • 免疫原

    Synthetic peptide:

    HHHHHH

    (6-His) conjugated to KLH.

  • 特記事項
    HIS-TAG® is a trademark of EMD Biosciences, Inc.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    PBS, pH 7.2, sodium azide, 0.1%
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab9136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/100 - 1/500.
ICC/IF Use at an assay dependent concentration. PubMed: 22247289
ICC 1/200 - 1/2000.
WB 1/1000 - 1/30000.

ターゲット情報

  • 関連性
    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 細胞内局在
    Depends upon the localization of the parent protein tagged with hexahistidine.
  • 別名
    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Anti-6X His tag® antibody 画像

  • ab9136, staining 6X His tag® in VMP001-conjugated nanoparticles by Immunocytochemistry/ Immunofluorescence. An AlexaFluor®488-conjugated anti-goat IgG was used as the secondary antibody.

Anti-6X His tag® antibody (ab9136) 使用論文

This product has been referenced in:
  • Fischer F  et al. Identification of potential mitochondrial CLPXP protease interactors and substrates suggests its central role in energy metabolism. Sci Rep 5:18375 (2015). WB . Read more (PubMed: 26679294) »
  • Fischer F  et al. RCF1-dependent respiratory supercomplexes are integral for lifespan-maintenance in a fungal ageing model. Sci Rep 5:12697 (2015). Read more (PubMed: 26220011) »

See all 4 Publications for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Liver)
Permeabilization
No
Specification
Liver
Blocking step
BSA as blocking agent for 8 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jul 26 2016

I'm afraid the antibody has not been tested for its ability to recognize internal epitopes, sorry about the inconvenience.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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