製品の概要

  • 製品名
    Anti-6X His tag® antibody
    6X His tag® 一次抗体 製品一覧
  • 製品の詳細
    Chicken polyclonal to 6X His tag®
  • 特異性
    Antibody specificity was verified by ELISA against peptide conjugated to BSA (HHHHHH-BSA). A 1:8000 dilution of the antibody gave an OD=1.0 in a 15 minute reaction using Goat Anti Chicken IgY (IgG) and TMB as the substrate. Appropriate specificity controls were run.
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 免疫原

    Synthetic peptide:

    HHHHHH

    conjugated to KLH.

  • 特記事項
    HIS-TAG® is a trademark of EMD Biosciences, Inc.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    PBS, pH 7.2, sodium azide, 0.1%
  • Concentration information loading...
  • 精製度
    IgY fraction
  • 特記事項(精製)
    Antibodies were immunoaffinity purified using the peptide immobilized on a solid support.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    unknown
  • 軽鎖の種類
    unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab9107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報
    This antibody was an effective at 1:1000 (1 µg/ml) dilution using an affinity purified goat anti chicken IgY (IgG) HRP.
    The Westerns probed with this antibody are very sharp and clear with extremely low background and no non-specific binding.
  • ターゲット情報

    • 関連性
      The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
    • 細胞内局在
      Depends upon the localization of the parent protein tagged with hexahistidine.
    • 別名
      • 6 His epitope tag antibody
      • Hexa His tag antibody
      • HHHHHH epitope tag antibody
      • HHHHHH tag antibody
      • His tag antibody
      • Polyhistidine Tag antibody
      see all

    プロトコール

    Anti-6X His tag® antibody (ab9107) 使用論文

    ab9107 has not yet been referenced specifically in any publications.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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